INHIBITION OF CRUZIPAIN, THE MAJOR CYSTEINE PROTEINASE OF THE PROTOZOAN PARASITE, TRYPANOSOMA-CRUZI, BY PROTEINASE-INHIBITORS OF THE CYSTATIN SUPERFAMILY

被引：77

作者：

STOKA, V

NYCANDER, M

LENARCIC, B

LABRIOLA, C

CAZZULO, JJ

BJORK, I

TURK, V

机构：

[1] SWEDISH UNIV AGR SCI, UPPSALA BIOMED CTR, DEPT VET MED CHEM, S-75123 UPPSALA, SWEDEN

[2] UNIV BUENOS AIRES, FAC CIENCIAS EXACTAS & NAT, CONICET, FDN CAMPOMAR, RA-1405 BUENOS AIRES, DF, ARGENTINA

来源：

FEBS LETTERS | 1995年 / 370卷 / 1-2期

关键词：

TRYPANOSOMA CRUZI; CRUZIPAIN; CYSTEINE PROTEINASE; CYSTATIN; INHIBITION; KINETICS;

D O I：

10.1016/0014-5793(95)00798-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cruzipain, the major cysteine proteinase from Trypanosoma cruzi epimastigotes, purified to a sequentially pure form, exists in multiple forms with pi values between 3.7 and 5.1, and an apparent molecular mass of 41 kDa, The enzyme is stable between pH 4.5-9.5, Cruzipain was found to be rapidly and tightly inhibited by various protein inhibitors of the cystatin superfamily (k(ass) = 1.7-79 x 10(6) M(-1)s(-1), K-d = 1.4-72 pM), These results suggest a possible defensive role for the host's cystatins after parasite infection, and may be of use for the design of new therapeutic drugs.

引用

页码：101 / 104

页数：4

共 35 条

[1] ALIGNMENT PHYLOGENY OF THE PAPAIN SUPERFAMILY OF CYSTEINE PROTEASES [J].