THE NEUROSPORA-CRASSA CHS-2 GENE ENCODES A NONESSENTIAL CHITIN SYNTHASE

被引：50

作者：

DIN, AB ^{[1
]}

YARDEN, O ^{[1
]}

机构：

[1] HEBREW UNIV JERUSALEM,FAC AGR,DEPT PLANT PATHOL & MICROBIOL,IL-76100 REHOVOT,ISRAEL

来源：

MICROBIOLOGY-UK | 1994年 / 140卷

关键词：

NEUROSPORA CRASSA; CELL-WALL BIOSYNTHESIS; MULTIPLE CHITIN SYNTHASES; POLYOXIN D;

D O I：

10.1099/13500872-140-9-2189

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Chitin is a structural component of morphologically distinct structures assembled during various phases of growth and development in filamentous fungi. In Neurospora crassa, at least three different DNA fragments related to chitin synthase have been identified. In this study we cloned, sequenced and characterized the chitin synthase 2 structural gene (designated chs-2). The amino acid sequence deduced from the cloned chs-2 genomic DNA fragments is very similar to that of chitin synthase genes isolated from other fungi. Inactivation of the N. crassa chs-2 gene by repeat-induced point (RIP) mutation produced progeny which under standard growth conditions were indistinguishable from the wild-type. However, a significant reduction in chitin synthase activity and increased sensitivity to the phosphatidylcholine biosynthesis inhibitor edifenphos are characteristic of the chs-2(RIP) strain.

引用

页码：2189 / 2197

页数：9

共 47 条

[21]

GOODAY GW, 1990, BIOCH CELL WALLS MEM, P61

[22] STRUCTURE AND DEVELOPMENT OF SEPTA IN NEUROSPORA-CRASSA [J].

HUNSLEY, D ;

GOODAY, GW .

PROTOPLASMA, 1974, 82 (1-2) :125-146

[23]

KANG MS, 1984, J BIOL CHEM, V259, P4966

[24] AN ANALYSIS OF 5'-NONCODING SEQUENCES FROM 699 VERTEBRATE MESSENGER-RNAS [J].

KOZAK, M .

NUCLEIC ACIDS RESEARCH, 1987, 15 (20) :8125-8148

[25] LOCALIZATION OF CHITIN SYNTHETASE IN CELL-FREE HOMOGENATES OF SACCHAROMYCES-CEREVISIAE - CHITOSOMES AND PLASMA-MEMBRANE [J].

LEALMORALES, CA ;

BRACKER, CE ;

BARTNICKIGARCIA, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (22) :8516-8520

[26] RELATIONSHIP OF MAJOR CONSTITUENTS OF NEUROSPORA CRASSA CELL WALL TO WILD-TYPE AND COLONIAL MORPHOLOGY [J].

MAHADEVA.PR ;

TATUM, EL .

JOURNAL OF BACTERIOLOGY, 1965, 90 (04) :1073-&

[27] IDENTIFICATION AND CHROMOSOMAL DISTRIBUTION OF 5S RIBOSOMAL-RNA GENES IN NEUROSPORA-CRASSA [J].

METZENBERG, RL ;

STEVENS, JN ;

SELKER, EU ;

MORZYCKAWROBLEWSKA, E .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (07) :2067-2071

[28]

MUZZARELLI RAA, 1986, CHITIN

[29] CLONING AND CHARACTERIZATION OF THE GENE FOR BETA-TUBULIN FROM A BENOMYL-RESISTANT MUTANT OF NEUROSPORA-CRASSA AND ITS USE AS A DOMINANT SELECTABLE MARKER [J].

ORBACH, MJ ;

PORRO, EB ;

YANOFSKY, C .

MOLECULAR AND CELLULAR BIOLOGY, 1986, 6 (07) :2452-2461

[30]

Peberdy JF, 1990, BIOCH CELL WALLS MEM, P5

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