A SYNTHETIC PEPTIDE SUBSTRATE FOR INITIATION FACTOR-II KINASES

被引：28

作者：

MELLOR, H

PROUD, CG

机构：

[1] Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol, BS8 1TD, University Walk

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 178卷 / 02期

关键词：

D O I：

10.1016/0006-291X(91)90125-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A peptide P(45-56) corresponding to residues 45-56 (sequence: ILLSELSRRRIR) of eIF-2α was synthesised. It was phosphorylated by both of the well characterised eIF-2α kinases viz.; the heme-controlled repressor (HCR) and the double stranded RNA-dependent inhibitor (dsI). Of four other protein kinases tested only protein kinase C (PKC) phosphorylated P(45-56), with complete dependence on phosphatidylserine. Only the residue corresponding to serine-51 in eIF-2α was phosphorylated by HCR, dsI or PKC. The phosphorylation of the peptide by dsI and the phosphorylation of eIF-2α by dsI or HCR showed sigmoidal kinetics with respect to substrate concentration. © 1991.

引用

页码：430 / 437

页数：8

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