RAPID CONTRASTING OF EXTRACELLULAR ELEMENTS IN THIN-SECTIONS

被引：42

作者：

DINGEMANS, KP ^{[1
]}

WEERMAN, MAV ^{[1
]}

机构：

[1] UNIV AMSTERDAM,ACAD MED CTR,DEPT PATHOL,MEIBERGDREEF 9,1105 AZ AMSTERDAM,NETHERLANDS

来源：

ULTRASTRUCTURAL PATHOLOGY | 1990年 / 14卷 / 06期

关键词：

Basement membrane; Collagen; Elastin; Electron microscopy; Staining procedure; Tannic acid;

D O I：

10.3109/01913129009076139

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

Standard methods for contrasting ultrathin sections generally have their greatest effect on cells and cellular components, whereas extracellular elements remain relatively electron-lucent. Occasionally, some extracellular elements even fail completely to react with the staining solutions. We describe a method for rendering a uniformly high contrast to extracellular tissue components. This consists of a brief prestaining of grids with diluted tannic acid in distilled water. Simple, rapid, and versatile, this procedure can be routinely applied to all tissue samples examined by electron microscopy. As an additional advantage, the method greatly enhances the electron density of intracellular glycogen. Higher concentrations of tannic acid give increased electron density, especially to elastin, and can therefore be used as an elastin stain. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：519 / 527

页数：9