RESISTANCE IN TRANSGENIC POTATO EXPRESSING THE POTATO LEAFROLL VIRUS COAT PROTEIN GENE

被引：79

作者：

KAWCHUK, LM

MARTIN, RR

MCPHERSON, J

机构：

[1] Research Station Agriculture Canada, Vancouver, BC V6T 1X2

[2] Department of Plant Science, University of British Columbia, Vancouver

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 1990年 / 3卷 / 05期

关键词：

Genetically engineered resistance; Tobacco;

D O I：

10.1094/MPMI-3-301

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The coat protein gene of potato leafroll virus (PLRV), a member of the luteovirus group, was inserted into potato via a gene transfer mediated by A grobacterium tumefaciens. Introduced as a chimeric gene under the control of the duplicated cauliflower mosaic virus 35S promoter, transcription levels were high but only low levels of coat protein were detected. Coat protein accumulation ranged from undetectable levels to a maximum of 0.01% of total leaf protein. Inoculation of transgenic plants with PLRV resulted in low virus titers that remained low or decreased indicating sustained resistance. High PLRV inoculation levels achieved with 25 viruliferous aphids did not overcome the resistance. This resistance will have practical applications for the control of PLRV and may also help to understand the mechanisms of virus infection. © 1990 The American Phytopathological Society.

引用

页码：301 / 307

页数：7

共 39 条

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