A PROMOTER ELEMENT OF THE HUMAN SERINE ESTERASE GRANZYME-B GENE CONTROLS SPECIFIC TRANSCRIPTION IN ACTIVATED T-CELLS

被引：28

作者：

HADDAD, P ^{[1
]}

WARGNIER, A ^{[1
]}

BOURGE, JF ^{[1
]}

SASPORTES, M ^{[1
]}

PAUL, P ^{[1
]}

机构：

[1] HOP ST LOUIS,CTR HAYEM,INSERM,U93,1 AVE CLAUDE VELLEFAUX,F-75010 PARIS,FRANCE

来源：

EUROPEAN JOURNAL OF IMMUNOLOGY | 1993年 / 23卷 / 03期

关键词：

T-CELL ACTIVATION; GENE REGULATION; HUMAN SERINE ESTERASE; GRANZYME-B;

D O I：

10.1002/eji.1830230307

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The human granzyme B gene encodes a serine protease expressed specifically in cytoplasmic granules of cytotoxic T lymphocytes. released upon effector-target cell interaction. Previous studies have shown that granzyme B mRNA was induced iii T lymphocytes after antigenic or mitogenic stimulation. To study the regulation of human granzyme B gene expression during lymphocyte activation we analyzed its 5' flanking region using chloramphenicol acetyl transferase (CAT) reporter gene constructs. We show that a 208-bp fragment (-148 to +60) containing an NF-AT (nuclear factor of activated T cells)-binding site promotes CAT expression in phytohemagglutinin-activated T lymphocytes, in immobilized monoclonal anti-CD3 antibody-activated Jurkat T cell line while it is inactive in unstimulated PEER and Jurkat T cells lines or B Epstein-Barr virus-transformed cell lines.

引用

页码：625 / 629

页数：5

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