DETECTION OF NEURON SPECIFIC ENOLASE CONCENTRATIONS IN CEREBROSPINAL-FLUID FROM PATIENTS WITH NEUROLOGICAL DISORDERS BY MEANS OF A SENSITIVE ENZYME-IMMUNOASSAY

被引：40

作者：

VERMUYTEN, K

LOWENTHAL, A

KARCHER, D

机构：

[1] Laboratory of Neurochemistry, Born-Bunge Foundation, U.I.A.

来源：

CLINICA CHIMICA ACTA | 1990年 / 187卷 / 02期

关键词：

Cerebrospinal fluid; Enzyme linked immunosorbent assay; Neurological disorder; Neuron-specific enolase;

D O I：

10.1016/0009-8981(90)90332-M

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

An enzyme linked immunosorbent assay (ELISA) for the detection of neuron specific enolase (NSE) in cerebrospinal fluid (CSF) was developed. The sensitivity of the ELISA was less than 1 μg/ml. This sensitivity is comparable with radioimmunoassays which have the disadvantage that radiolabelled products are used. The developed assay was used to measure cerebrospinal fluid neuron specific enolase (CSF-NSE) levels in 1178 patients with neurological disorders to establish its potential usefulness and clinical application. CSF-NSE levels in this group of patients were independent of sex and no correlation with age was found. CSF-NSE was significantly increased in Creutzfeldt-Jacob disease, meningeal hemorrhage, thrombosis, Guillain-Barré syndrome and in schizophrenia. © 1990.

引用

页码：69 / 78

页数：10

共 40 条

[1] S-100 PROTEIN AND NEURON-SPECIFIC ENOLASE IN AMNIOTIC-FLUID AS MARKERS OF ABDOMINAL-WALL AND NEURAL-TUBE DEFECTS IN THE FETUS [J].