PLANT-METABOLISM AS AN ANALYTICAL TOOL - SOME APPLICATIONS OF PLANT-TISSUE ELECTRODES FOR THE SELECTIVE DETERMINATION OF CATECHOLAMINES

被引：32

作者：

MAZZEI, F ^{[1
]}

BOTRE, F ^{[1
]}

LANZI, M ^{[1
]}

LORENTI, G ^{[1
]}

PORCELLI, F ^{[1
]}

BOTRE, C ^{[1
]}

机构：

[1] UNIV LA SAPIENZA,IST MERCEOL,I-00161 ROME,ITALY

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 1992年 / 7卷 / 1-3期

关键词：

D O I：

10.1016/0925-4005(92)80337-W

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The present work deals with the quantitative determination of catechol and other biologically active polyphenols (catecholamines) by means of plant tissue electrodes built up by a whole tissue containing an oxidizing enzyme (polyphenol oxidase, PPO) coupled with an O2 gas-selective electrode. Several tissues have been employed to prepare the biocatalytic layer of the biosensors. The analytical characteristics of the plant tissue electrodes here proposed, tested in catechol standard solutions, are comparable to those of traditional PPO-based enzyme electrodes. Moreover, the whole-tissue biocatalytic membranes are more stable than those prepared by using purified enzymes, thus ensuring longer life times of application. The biosensors presented here are also suitable for the quantitative determination of other catecholamines (epinephrine, norepinephrine, dopamine, L-DOPA),

引用

页码：427 / 430

页数：4