BILAYER-LIPID MEMBRANES FOR FLOW-INJECTION MONITORING OF ACETYLCHOLINE, UREA, AND PENICILLIN

被引:72
作者
NIKOLELIS, DP
SIONTOROU, CG
机构
[1] Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Athens, Panepistimiopolis-Kouponia
关键词
D O I
10.1021/ac00101a022
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
This work describes a technique for the rapid and sensitive determination of acetylcholine, urea, and penicillin in flowing solution streams using stabilized systems of solventless bilayer lipid membranes (BLMs). This method of monitoring substrates of hydrolytic enzyme reactions made use of BLMs which were supported on ultrafiltration membranes such as polycarbonate and glass microfiber; these filter membranes were found to enhance the stability of BLMs for uses in flow injection experiments, The enzymes were immobilized on BLMs by incorporating the protein solution into the lipid matrix at the air/electrolyte interface before the BLM formation, followed by injections of the substrates into flowing streams of a carrier electrolyte solution, Hydronium ions produced by the enzymatic reaction at the BLM surface caused dynamic alterations of the electrostatic fields and phase structure of BLMs, and as a result ion current transients were obtained; the magnitude of these signals was correlated to the substrate concentration, which could be determined at the micromolar level. The response times were ca, 10 s, and acetylcholine, urea, and penicillin could be determined in continuous flowing systems with a maximum rate of 220 samples/h. It is expected that this analytical utility of stabilized BLMs for flow stream uses will provide new opportunities in this strategy of chemical sensing.
引用
收藏
页码:936 / 944
页数:9
相关论文
共 52 条
[31]  
NIKOLELIS DP, IN PRESS J ELECTROAN
[32]   ABSORPTION BEHAVIORS OF SURFACTANT MOLECULES ON A LIPID-COATED QUARTZ-CRYSTAL MICROBALANCE - AN ALTERNATIVE TO EYE-IRRITANT TESTS [J].
OKAHATA, Y ;
EBATO, H .
ANALYTICAL CHEMISTRY, 1991, 63 (03) :203-207
[33]   SYNTHETIC CHEMORECEPTIVE MEMBRANES - SENSING BITTER OR ODOROUS SUBSTANCES ON A SYNTHETIC LIPID MULTIBILAYER FILM BY USING QUARTZ-CRYSTAL MICROBALANCES AND ELECTRIC RESPONSES [J].
OKAHATA, Y ;
ENNA, G ;
EBATO, H .
ANALYTICAL CHEMISTRY, 1990, 62 (14) :1431-1438
[34]   HYDROGEN-PEROXIDE OXYGEN BIOSENSOR BASED ON SUPPORTED PHOSPHOLIPID-BILAYER [J].
OTTO, M ;
SNEJDARKOVA, M ;
REHAK, M .
ANALYTICAL LETTERS, 1992, 25 (04) :653-662
[36]   DESIGN OF A GLUCOSE MINISENSOR BASED ON STREPTAVIDIN GLUCOSE-OXIDASE COMPLEX COUPLING WITH SELF-ASSEMBLED BIOTINYLATED PHOSPHOLIPID MEMBRANE ON SOLID SUPPORT [J].
SNEJDARKOVA, M ;
REHAK, M ;
OTTO, M .
ANALYTICAL CHEMISTRY, 1993, 65 (06) :665-668
[37]  
STRYER L, 1988, BIOCHEMISTRY-US, pCH8
[38]   NA+/D-GLUCOSE COTRANSPORTER BASED BILAYER LIPID-MEMBRANE SENSOR FOR D-GLUCOSE [J].
SUGAO, N ;
SUGAWARA, M ;
MINAMI, H ;
UTO, M ;
UMEZAWA, Y .
ANALYTICAL CHEMISTRY, 1993, 65 (04) :363-369
[39]  
TAYLOR P, 1980, PHARM BASIS THERAPEU, pCH6
[40]   STRUCTURE AND ELECTROCHEMICAL PROPERTIES OF MICRO-FILTRATION FILTER LIPID-MEMBRANE SYSTEMS [J].
THOMPSON, M ;
LENNOX, RB ;
MCCLELLAND, RA .
ANALYTICAL CHEMISTRY, 1982, 54 (01) :76-81