GENOMIC SCANNING FOR EXPRESSED SEQUENCES IN XP21 IDENTIFIES THE GLYCEROL KINASE GENE

被引：45

作者：

GUO, WW

WORLEY, K

ADAMS, V

MASON, J

SYLVESTERJACKSON, D

ZHANG, YH

TOWBIN, JA

FOGT, DD

MADU, S

WHEELER, DA

MCCABE, ERB

机构：

[1] BAYLOR COLL MED,INST MOLEC GENET,HOUSTON,TX 77030

[2] BAYLOR COLL MED,DEPT PEDIAT,HOUSTON,TX 77030

[3] BAYLOR COLL MED,DEPT CELL BIOL,HOUSTON,TX 77030

来源：

NATURE GENETICS | 1993年 / 4卷 / 04期

关键词：

D O I：

10.1038/ng0893-367

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Rapid genomic scanning methods are required to identify expressed sequences and we report an efficient, sensitive and specific approach which relies upon hybridization of an amplified, labeled cDNA library to digested cosmid DNA. We identified expressed sequences within a cosmid in the glycerol kinase (GK) ''critical region'' of Xp21 that had impressive similarity to prokaryotic GKs. We used this genomic sequence information to clone the human hepatic GK cDNA. Independent confirmation of the identity of this gene was obtained by functional complementation of GK deficient E. coli mutants with a construct containing the complete human X-linked GK coding sequence.

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页码：367 / 372

页数：6

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