COMBINED USE OF RFLP AND PCR-ASO-TYPING FOR HLA-DR-DW-TYPING AND DQW-TYPING

被引：5

作者：

BIGNON, JD ^{[1
]}

BIDWELL, JL ^{[1
]}

机构：

[1] UNITED KINGDOM TRANSPLANT SERV, MOLEC GENET LAB, BRISTOL, ENGLAND

来源：

EUROPEAN JOURNAL OF IMMUNOGENETICS | 1991年 / 18卷 / 1-2期

关键词：

D O I：

10.1111/j.1744-313X.1991.tb00012.x

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Due to some limitations of restriction fragment length polymorphism (RFLP) analysis in HLA-DR-DQ typing, we present a combined use of RFLP and polymerase chain reaction (PCR)-allele-specific oligonucleotide (ASO) typing. This scheme consists in selectively amplifying the few RFLP ill-defined genes (DR1/DR'Br' and DR4-Dw subsets) using PCR with allele specific primers to avoid cross-hybridization.

引用

页码：125 / 138

页数：14

共 80 条

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