NUCLEOTIDE-SEQUENCE OF A PSEUDOMONAS-DENITRIFICANS 5.4-KILOBASE DNA FRAGMENT CONTAINING 5 COB GENES AND IDENTIFICATION OF STRUCTURAL GENES ENCODING S-ADENOSYL-L-METHIONINE - UROPORPHYRINOGEN-III METHYLTRANSFERASE AND COBYRINIC ACID A,C-DIAMIDE SYNTHASE

被引：64

作者：

CROUZET, J ^{[1
]}

CAUCHOIS, L ^{[1
]}

BLANCHE, F ^{[1
]}

DEBUSSCHE, L ^{[1
]}

THIBAUT, D ^{[1
]}

ROUYEZ, MC ^{[1
]}

RIGAULT, S ^{[1
]}

MAYAUX, JF ^{[1
]}

CAMERON, B ^{[1
]}

机构：

[1] RHONE POULENC SANTE,CTR RECH VITRY,DEPT CHIM ANALYT,F-94403 VITRY,FRANCE

来源：

JOURNAL OF BACTERIOLOGY | 1990年 / 172卷 / 10期

关键词：

D O I：

10.1128/jb.172.10.5968-5979.1990

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A 5.4-kilobase DNA fragment carrying Pseudomonas dentrificans cob genes has been sequenced. The nucleotide sequence and genetic analysis revealed that this fragment carries five different cob genes (cobA to cobE). Four of these genes present the characteristics of translationally coupled genes. cobA has been identified as the structural gene of S-adenosyl-L-methionine:uroporphyrinogen III methyltransferase (SUMT) because the encoded protein has the same NH2 terminus and molecular weight as those determined for the purified SUMT. For the same reasons the cobB gene was shown to be the structural gene for cobyrinic acid a,c-diamide synthase. Genetic and biochemical data concerning cobC and cobD mutants suggest that the products of these genes are involved in the conversion of cobyric acid to cobinamide.

引用

页码：5968 / 5979

页数：12

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