MASS MEASUREMENT ACCURACY OF MATRIX-ASSISTED LASER-DESORBED BIOMOLECULES - A FOURIER-TRANSFORM ION-CYCLOTRON RESONANCE MASS-SPECTROMETRY STUDY

被引：28

作者：

SOLOUKI, T ^{[1
]}

GILLIG, KJ ^{[1
]}

RUSSELL, DH ^{[1
]}

机构：

[1] TEXAS A&M UNIV, DEPT CHEM, COLL STN, TX 77843 USA

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1994年 / 8卷 / 01期

关键词：

D O I：

10.1002/rcm.1290080106

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of binary matrices and internal calibrants to improve the mass measurement accuracy in matrix-assisted laser desorption ionization (MALDI) with Fourier-transform ion cyclotron resonance (FTICR) mass spectrometry is described. Binary matrices enhance the analyte ion yield and enable a complete MALDI-FTICR mass spectrum to be obtained from a single laser shot. The advantage of single-laser-shot data acquisition is that it eliminates line-broadening due to shot-to-shot frequency variations. It is shown that unresolved product ions, mainly due to loss of H2O and/or NH3, shift the centroid of an unresolved multi-component peak. A mass measurement accuracy of 12 ppm was obtained for the bovine insulin [M + H]+ ion using melittin as an internal calibrant.

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页码：26 / 31

页数：6

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