ONLINE LIQUID-CHROMATOGRAPHY THERMOSPRAY MASS-SPECTROMETRY IN THE ANALYSIS OF OLIGOSACCHARIDES

被引：20

作者：

NIESSEN, WMA ^{[1
]}

VANDERHOEVEN, RAM ^{[1
]}

VANDERGREEF, J ^{[1
]}

SCHOLS, HA ^{[1
]}

VORAGEN, AGJ ^{[1
]}

机构：

[1] WAGENINGEN UNIV AGR,DEPT FOOD SCI,6703 HD WAGENINGEN,NETHERLANDS

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1992年 / 6卷 / 03期

关键词：

D O I：

10.1002/rcm.1290060309

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The analysis of intact neutral oligosaccharides by on-line liquid chromatography/thermospray mass spectrometry is described. Molecular-weight information on oligomers up to a degree of polymerization of 10 is obtained using an aqueous mobile phase containing 10(-4) mol/L sodium acetate, which was found to be compatible with thermospray interfacing and ionization. Ions due to sodiated and disodiated oligosaccharides are observed under these conditions without fragmentation. The aqueous 10(-4) mol/L sodium acetate mobile phase is demonstrated to be applicable in the separation of mixtures of oligosaccharides on a reversed-phase octadecyl-modified silica column.

引用

页码：197 / 202

页数：6

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