EFFECTS OF MATERNAL CAFFEINE INTAKE DURING LACTATION ON MOLAR ENAMEL SURFACES IN NEWBORN RATS

被引:7
作者
HASHIMOTO, K
JOSEPH, F
FALSTER, AU
SIMMONS, WB
NAKAMOTO, T
机构
[1] LOUISIANA STATE UNIV,MED CTR,DEPT PHYSIOL,NEW ORLEANS,LA 70119
[2] LOUISIANA STATE UNIV,MED CTR,DEPT PEDIAT,PERINATAL NUTR & METAB LAB,NEW ORLEANS,LA 70119
[3] UNIV NEW ORLEANS,DEPT GEOL & GEOPHYS,NEW ORLEANS,LA 70148
关键词
CAFFEINE; ENAMEL SURFACE; NEWBORN RATS;
D O I
10.1016/0003-9969(92)90005-S
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Dams were fed normal laboratory chow until delivery. At birth, the litters were combined, and eight pups were randomly assigned to each dam. Dams with the recombined litters were divided into two groups. Dams of group 1 were fed a 20% protein diet as a control; dams of group 2 were fed a 20% protein diet supplemented with caffeine (2 mg/100 g of the dam's weight). On day 22, the dams of group 2 were anaesthetized with ether. They were injected with 2 iu of oxytocin in order to collect milk. Blood was collected from pups and dams to determine its caffeine concentration. The first and second molars were removed from each pup's mandible and maxilla. Radiographs were taken of 10 randomly selected first or second molars from each group. Four randomly selected molars from each litter were placed in a specially designed chamber and bathed with a constant flow of acid solution to determine the amount of mineral dissolved from the enamel surfaces. The remaining non-acid exposed molars were pulverized in freezer mills. A small portion of this powder was then analysed for the total amount of minerals. No differences were found in the radiographic density of enamel between the groups. The amount of dissolved calcium, phosphorus and magnesium from enamel surfaces in the caffeine group was consistently greater than that of the non-caffeine group in the first molars, whereas, in the second molars, there was no difference between the groups. The concentration of calcium, phosphorus and zinc in the first molars in the caffeine group was less than that in the non-caffeine group, whereas there was no difference between the groups in the second molars. Thus the caffeine intake of suckling pups in the early growing period affected the enamel surface of the first molar.
引用
收藏
页码:105 / 109
页数:5
相关论文
共 20 条
[1]   CAFFEINE - A TOXICOLOGICAL OVERVIEW [J].
ABBOTT, PJ .
MEDICAL JOURNAL OF AUSTRALIA, 1986, 145 (10) :518-521
[2]   EFFECT OF CAFFEINE ON RAT OFFSPRING FROM TREATED DAMS [J].
AESCHBACHER, HU ;
MILON, H ;
POOT, A ;
WURZNER, HP .
TOXICOLOGY LETTERS, 1980, 7 (01) :71-77
[3]  
Aldridge A, 1975, CLIN PHARMACOL THER, V25, P977
[4]   PRE-ERUPTIVE PROTEIN-ENERGY MALNUTRITION AND ACID SOLUBILITY OF RAT MOLAR ENAMEL SURFACES [J].
APONTEMERCED, I ;
NAVIA, JM .
ARCHIVES OF ORAL BIOLOGY, 1980, 25 (11-1) :701-705
[5]   EFFECT OF ZINC-DEFICIENCY ON DENTAL-CARIES [J].
BROWN, ED ;
CALHOUN, NR ;
LARSON, RH ;
SMITH, JC .
LIFE SCIENCES, 1979, 24 (22) :2093-2097
[6]   MICRODETERMINATION OF PHOSPHORUS [J].
CHEN, PS ;
TORIBARA, TY ;
WARNER, H .
ANALYTICAL CHEMISTRY, 1956, 28 (11) :1756-1758
[7]  
GILBERT EF, 1973, J REPROD FERTIL, V34, P494
[8]  
GRAHAM DM, 1978, NUTR REV, V36, P97, DOI 10.1111/j.1753-4887.1978.tb03717.x
[9]   DEVELOPMENTAL-CHANGES IN COMPOSITION OF RAT MILK - TRACE-ELEMENTS, MINERALS, PROTEIN, CARBOHYDRATE AND FAT [J].
KEEN, CL ;
LONNERDAL, B ;
CLEGG, M ;
HURLEY, LS .
JOURNAL OF NUTRITION, 1981, 111 (02) :226-236
[10]  
Kleiber M., 1961, FIRE LIFE INTRO ANIM, P177