EVALUATION OF THE ENANTIOSELECTIVITY OF AN OVOMUCOID AND A CELLULASE CHIRAL STATIONARY PHASE TOWARDS A SET OF BETA-BLOCKING-AGENTS

The enantioselectivity of chiral stationary phases (CSP) with ovomucoid and cellulase proteins as chiral selector, towards twenty-four beta-blocking agents has been evaluated. On both phases, influences of hydrophobicity, mobile phase modifier content and mobile phase pH on retention behaviour and enantioselectivity were studied. Compounds with a value of the logarithm of the partition coefficient between n-octanol and water (log P) smaller than 2, are not likely to be enantioseparated on ovomucoid. For the cellulase CSP, a correlation was found between retention behaviour and hydrophobicity. This CSP showed very good enantioselectivity towards the group of compounds studied and a trend in higher separation coefficients for more hydrophobic compounds was observed.