DONOR LEUKOCYTE MIGRATION FOLLOWING EXTREMITY TRANSPLANTATION IN AN EXPERIMENTAL-MODEL

被引:18
作者
HOFFMAN, LA
CODNER, MA
SHUSTER, BA
BOGOSSIAN, N
EGOZI, L
HARPER, AD
STEINMAN, RM
LATRENTA, GS
SHIRES, GT
机构
[1] CORNELL UNIV, MED CTR,NEW YORK HOSP,DEPT SURG, DIV PLAST & RECONSTRUCT SURG, NEW YORK, NY 10021 USA
[2] ROCKEFELLER UNIV, CELLULAR PHYSIOL & IMMUNOL LAB, NEW YORK, NY 10021 USA
关键词
D O I
10.1097/00006534-199212000-00010
中图分类号
R61 [外科手术学];
学科分类号
摘要
In an effort to further define the immunologic mechanisms leading to acute composite-tissue allograft rejection, the migratory patterns of donor leukocytes were evaluated. Using a rat model, 52 orthotopic vascularized hindlimb transplants were performed in strains representing major histocompatibility mismatches. In order to evaluate the effect of allogeneic skin on limb rejection, all donor skin was removed in a second group of allografts. Recipient lymphoid organs were examined during the week following transplantation for antigen-presenting cells using a donor-specific class II monoclonal antibody. Donor leukocytes, with dendritic cell morphology, were identified in recipient spleen and lymph nodes draining the allograft. Significantly higher numbers of donor leukocytes were present during postoperative days 1 through 4 for both groups. Association of these important passenger leukocytes with host T-lymphocytes may represent the site of initiation of the immune response.
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页码:999 / 1006
页数:8
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