PHOSPHOENOLPYRUVATE CARBOXYKINASE AND GLUCOSE-6-PHOSPHATE-DEHYDROGENASE EXPRESSION IN FETAL HEPATOCYTE PRIMARY CULTURES UNDER PROLIFERATIVE CONDITIONS

被引：16

作者：

MOLERO, C ^{[1
]}

VALVERDE, AM ^{[1
]}

BENITO, M ^{[1
]}

LORENZO, M ^{[1
]}

机构：

[1] UNIV COMPLUTENSE MADRID,FAC FARM,CSIC,CTR MIXTO,DEPT BIOQUIM & BIOL MOLEC,CIUDAD UNIV,E-28040 MADRID,SPAIN

来源：

EXPERIMENTAL CELL RESEARCH | 1992年 / 200卷 / 02期

关键词：

D O I：

10.1016/0014-4827(92)90175-8

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Fetal hepatocytes cultured for 64 h in the presence of glucagon and dexamethasone maintain a quiescent state, showing a low expression of glucose-6-phosphate dehydrogenase (G6PD) and a high induction of phosphoenolpyruvate carboxykinase (PEPCK). Under these culture conditions, the presence of EGF produced hepatocyte proliferation, with a concomitant increase of DNA synthesis, DNA content, and G6PD expression, meanwhile the expression of PEPCK was drastically reduced. The presence of forskolin plus IBMX nearly suppressed the increase in DNA synthesis and G6PD expression induced by EGF, showing a very high expression of PEPCK. Accordingly, it is possible to establish an inverse relation between G6PD, highly expressed in proliferating fetal hepatocytes, and PEPCK expression, highly expressed in quiescent fetal hepatocytes under specific hormonal stimulation. © 1992.

引用

页码：295 / 300

页数：6

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