MOLECULAR-CLONING AND CHARACTERIZATION OF A CDNA SHOWING TUMOR-SUPPRESSIVE ACTIVITY IN V-SRC-TRANSFORMED 3Y1 RAT FIBROBLASTS

被引：3

作者：

SAKIYAMA, S

OZAKI, T

ENOMOTO, H

机构：

[1] Division of Biochemistry, Chiba Cancer Center Research Institute, Chuoh-ku, Chiba, 260, 666-2, Nitona

来源：

ADVANCES IN ENZYME REGULATION, VOL 34 | 1994年 / 34卷

关键词：

V-SRC; TRANSFORMATION; RAT FIBROBLAST; DIFFERENTIAL HYBRIDIZATION; CDNA; DNA SEQUENCE; GENE EXPRESSION; DOWN-REGULATION; ONCOGENE(S); SUPPRESSOR GENE(S); TRANSFECTION; TUMORIGENICITY; COLONY FORMATION; EXPRESSION VECTOR; NEOMYCIN; NORTHERN ANALYSIS;

D O I：

10.1016/0065-2571(94)90019-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Differential screening procedure was employed to isolate new genes specifically down-regulated in RSV-transformed rat 3Y1 cells (SR-3Y1) compared with parental 3Y1 cells. Among those, one clone termed N03 showed nearly complete down-regulation in v-src-, v-mos and SV40-transformed 3Y1 cells. Nucleotide sequence analysis showed that N03 protein is novel and is composed of 178 amino acid residues in length. Introduction of N03 cDNA into SR-3Y1 cells using an expression vector resulted in suppression of transformed phenotypes such as high growth rate, colony-forming ability in soft agar and tumorigenicity in nude mice. © 1994.

引用

页码：247 / 255

页数：9

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