PLASTICITY OF MATURE SENSORY CEREBROVASCULAR AXONS FOLLOWING INTRACRANIAL INFUSION OF NERVE GROWTH-FACTOR

被引:27
作者
ISAACSON, LG [1 ]
ONDRIS, D [1 ]
CRUTCHER, KA [1 ]
机构
[1] UNIV CINCINNATI,DEPT NEUROSURG,CINCINNATI,OH 45267
关键词
NEURONAL PLASTICITY; ELECTRON MICROSCOPY; CEREBROVASCULAR INNERVATION; CGRP IMMUNOHISTOCHEMISTRY;
D O I
10.1002/cne.903610309
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Mature perivascular sympathetic axons associated with the intradural segment of the internal carotid artery (ICA) of the adult rat respond by sprouting following a two week infusion of nerve growth factor (NGF) into the lateral ventricle of the brain. Because nonsympathetic axons such as those comprising the sensory and parasympathetic population have been shown to respond to NGF, the present study was carried out to determine whether mature sensory axons respond to in vivo NGF infusion and whether competitive interactions between the innervating populations might affect the responsiveness of these axons to NGF. Standard electron microscopic techniques as well as calcitonin-gene-related peptide (CGRP) immunohistochemistry at the light microscopic level were used to examine the effects of intracerebroventricular NGF infusion on mature perivascular fibers with and without prior sympathetic denervation (i.e., bilateral superior cervical ganglionectomy). Following NGF infusion, CGRP-immunoreactive fibers appeared thicker and more numerous in the longitudinal plane when compared with vehicle controls. However, at the ultrastructural level, a significant increase in the total number of axons was not observed, although there was an increase in the number of large granular vesicles, suggesting that the CGRP fibers responded to exogenous NGF with an increase in neurotransmitter content, but not by sprouting. Sympathetic denervation, on the other hand, resulted in a significant increase in the number of fibers passing in the circumferential plane. The most dramatic change in CORP immunoreactivity was observed following combined sympathetic denervation and subsequent NGF infusion, where, in addition to the presence of thicker immunoreactive fibers, a significant increase in the perivascular density of immunoreactive fibers associated with the intradural blood vessels was observed. These findings suggest that exogenous NGF has different effects on mature sympathetic and nonsympathetic fibers that innervate intradural blood vessels. The former exhibit robust sprouting, whereas the latter do not sprout in response to NGF but show evidence for increased neuropeptide content. In addition, the heightened response by sensory axons following denervation and subsequent NGF infusion provides support for the idea that sensory and sympathetic axons normally compete for target space and/or target-derived neurotrophic factors. (C) 1995 Wiley-Liss, Inc.
引用
收藏
页码:451 / 460
页数:10
相关论文
共 56 条
[1]   MARKED INCREASES IN CALCITONIN GENE-RELATED PEPTIDE-CONTAINING NERVES IN THE DEVELOPING RAT FOLLOWING LONG-TERM SYMPATHECTOMY WITH GUANETHIDINE [J].
ABERDEEN, J ;
CORR, L ;
MILNER, P ;
LINCOLN, J ;
BURNSTOCK, G .
NEUROSCIENCE, 1990, 35 (01) :175-184
[2]   GUANETHIDINE SYMPATHECTOMY OF MATURE RATS LEADS TO INCREASES IN CALCITONIN GENE-RELATED PEPTIDE AND VASOACTIVE INTESTINAL POLYPEPTIDE-CONTAINING NERVES [J].
ABERDEEN, J ;
MILNER, P ;
LINCOLN, J ;
BURNSTOCK, G .
NEUROSCIENCE, 1992, 47 (02) :453-461
[3]  
ANDREWS TJ, 1994, J NEUROSCI, V14, P3048
[4]   THE TRK FAMILY OF NEUROTROPHIN RECEPTORS [J].
BARBACID, M .
JOURNAL OF NEUROBIOLOGY, 1994, 25 (11) :1386-1403
[5]   TROPHIC FACTORS AND NEURONAL SURVIVAL [J].
BARDE, YA .
NEURON, 1989, 2 (06) :1525-1534
[6]   SHORT-TERM AND LONG-TERM EFFECTS OF NERVE GROWTH-FACTOR ON SYMPATHETIC NERVOUS-SYSTEM IN ADULT MOUSE [J].
BJERRE, B ;
BJORKLUND, A ;
MOBLEY, W ;
ROSENGREN, E .
BRAIN RESEARCH, 1975, 94 (02) :263-277
[7]   NERVE GROWTH-FACTOR REVISITED [J].
BRADSHAW, RA ;
BLUNDELL, TL ;
LAPATTO, R ;
MCDONALD, NQ ;
MURRAYRUST, J .
TRENDS IN BIOCHEMICAL SCIENCES, 1993, 18 (02) :48-52
[8]   USE OF ENHANCED SILVER STAINING COMBINED WITH ELECTRON-MICROSCOPIC IMMUNOLABELLING TO DEMONSTRATE THE COLOCALIZATION OF NEUROPEPTIDE-Y AND VASOACTIVE INTESTINAL POLYPEPTIDE IN CEREBROVASCULAR NERVES [J].
CAVANAGH, JFR ;
MIONE, MC ;
BURNSTOCK, G .
NEUROSCIENCE, 1990, 39 (03) :775-785
[9]   THE P75 NEUROTROPHIN RECEPTOR [J].
CHAO, MV .
JOURNAL OF NEUROBIOLOGY, 1994, 25 (11) :1373-1385
[10]   CALCITONIN GENE-RELATED PEPTIDE AND CEREBRAL BLOOD-VESSELS - DISTRIBUTION AND VASOMOTOR EFFECTS [J].
EDVINSSON, L ;
EKMAN, R ;
JANSEN, I ;
MCCULLOCH, J ;
UDDMAN, R .
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 1987, 7 (06) :720-728