DELETION OF THE PRC (TSP) GENE PROVIDES EVIDENCE FOR ADDITIONAL TAIL-SPECIFIC PROTEOLYTIC ACTIVITY IN ESCHERICHIA-COLI K-12

被引：35

作者：

SILBER, KR ^{[1
]}

SAUER, RT ^{[1
]}

机构：

[1] MIT,DEPT BIOL,CAMBRIDGE,MA 02139

来源：

MOLECULAR & GENERAL GENETICS | 1994年 / 242卷 / 02期

关键词：

SELECTIVE PROTEIN DEGRADATION; PRC (TSP) DELETION STRAIN; CARBOXYL TERMINUS; LAMBDA REPRESSOR; ESCHERICHIA COLI K-12;

D O I：

10.1007/BF00391018

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Escherichia coli protease Pre (Tsp) exhibits specificity in vitro for proteins with nonpolar carboxyl termini. To determine whether Pre is responsible for the selective degradation in vivo of proteins with nonpolar carboxyl termini, we constructed a pre (tsp) deletion strain. Deletion of the pre gene did not prevent the rapid intracellular degradation of a variant of the amino-terminal domain of lambda repressor with a nonpolar carboxyl terminus, even though this protein is a substrate for Pre in vitro. Our results indicate that at least one additional carboxy-terminal-specific proteolytic system must exist in E. coli.

引用

页码：237 / 240

页数：4

共 11 条

[1]

BOWIE JU, 1989, J BIOL CHEM, V264, P7596

[2] CLONING, MAPPING, AND CHARACTERIZATION OF THE ESCHERICHIA-COLI PRC GENE, WHICH IS INVOLVED IN C-TERMINAL PROCESSING OF PENICILLIN-BINDING PROTEIN-3 [J].