CONFIRMATION OF THE HUMAN CATHEPSIN-B GENE (CTSB) ASSIGNMENT TO CHROMOSOME-8

被引：39

作者：

FONG, D

CHAN, MMY

HSIEH, WT

MENNINGER, JC

WARD, DC

机构：

[1] UNIV ALABAMA,DEPT BIOL,BIRMINGHAM,AL 35294

[2] NIMH,CLIN NEUROGENET BRANCH,BETHESDA,MD 20892

[3] YALE UNIV,SCH MED,DEPT HUMAN GENET,NEW HAVEN,CT 06510

[4] RUTGERS STATE UNIV,DEPT BIOL SCI,PISCATAWAY,NJ 08855

来源：

HUMAN GENETICS | 1992年 / 89卷 / 01期

关键词：

D O I：

10.1007/BF00207033

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Human cathepsin B gene (CTSB) has been mapped to two locations: 8p22 and 13q14. Here we confirm the chromosome 8 assignment by three independent methods: (1) analysis of human-hamster somatic cell hybrid DNA by polymerase chain reaction; (2) comparison of hybridization signals to cathepsin B in interphase nuclei of normal fibroblasts and fibroblasts with a chromosome 8 deletion; and (3) fluorescence in situ hybridization to metaphase spreads using cathepsin B cosmid clones. Our results indicate that human CTSB is located at 8p22-p23.1.

引用

页码：10 / 12

页数：3

共 21 条

[1] CLONING AND SEQUENCING OF THE 52K CATHEPSIN-D COMPLEMENTARY DEOXYRIBONUCLEIC-ACID OF MCF7 BREAST-CANCER CELLS AND MAPPING ON CHROMOSOME-11
AUGEREAU, P
GARCIA, M
MATTEI, MG
CAVAILLES, V
DEPADOVA, F
DEROCQ, D
CAPONY, F
FERRARA, P
ROCHEFORT, H
[J]. MOLECULAR ENDOCRINOLOGY, 1988, 2 (02) : 186 - 192
[2] IMPROVEMENT OF PCR AMPLIFIED DNA SEQUENCING WITH THE AID OF DETERGENTS
BACHMANN, B
LUKE, W
HUNSMANN, G
[J]. NUCLEIC ACIDS RESEARCH, 1990, 18 (05) : 1309 - 1309
[3] INSITU HYBRIDIZATION BANDING OF HUMAN-CHROMOSOMES WITH ALU-PCR PRODUCTS - A SIMULTANEOUS KARYOTYPE FOR GENE-MAPPING STUDIES
BALDINI, A
WARD, DC
[J]. GENOMICS, 1991, 9 (04) : 770 - 774
[4] BOUYON C, 1989, CYTOGENET CELL GENET, V51, P966
[5] EXPRESSION OF HUMAN CATHEPSIN-B PROTEIN IN ESCHERICHIA-COLI
CHAN, MM
FONG, D
[J]. FEBS LETTERS, 1988, 239 (02) : 219 - 222
[6] NUCLEOTIDE AND PREDICTED AMINO-ACID-SEQUENCES OF CLONED HUMAN AND MOUSE PREPROCATHEPSIN-B CDNAS
CHAN, SJ
SANSEGUNDO, B
MCCORMICK, MB
STEINER, DF
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (20) : 7721 - 7725
[7] ASSIGNMENT OF CATHEPSIN-E (CTSE) TO HUMAN-CHROMOSOME REGION 1Q31 BY INSITU HYBRIDIZATION AND ANALYSIS OF SOMATIC-CELL HYBRIDS
COUVREUR, JM
AZUMA, T
MILLER, DA
ROCCHI, M
MOHANDAS, TK
BOUDI, FA
TAGGART, RT
[J]. CYTOGENETICS AND CELL GENETICS, 1990, 53 (2-3): : 137 - 139
[8] DENEKAMP D S, 1985, American Journal of Human Genetics, V37, pA229
[9] EVANS GA, 1987, METHOD ENZYMOL, V152, P604
[10] FAN YS, 1989, CYTOGENET CELL GENET, V51, P996

← 1 2 3 →