IDENTIFICATION OF PEAKS IN CAPILLARY ZONE ELECTROPHORESIS BASED ON ACTUAL MOBILITIES

被引：29

作者：

VESPALEC, R ^{[1
]}

GEBAUER, P ^{[1
]}

BOCEK, P ^{[1
]}

机构：

[1] CZECHOSLOVAK ACAD SCI, INST ANALYT CHEM, VEVERI 97, CS-61142 BRNO, CZECHOSLOVAKIA

来源：

ELECTROPHORESIS | 1992年 / 13卷 / 9-10期

关键词：

D O I：

10.1002/elps.11501301144

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A procedure is proposed for the calculation of the actual effective mobility of a zone from its migration time. It is based on the use of internal standards with known mobilities; the use of two internal standards provides reliable mobility data even if the magnitudes of the effects of sample composition, capillary temperature, capillary length, migration distance, used voltage, as well as the tube length occupied by the injected sample are unknown. Formulas have been derived for the calculation of the actual mobilities, and their experimental verification has been carried out by using a model set of anionic solutes with mobilities ranging from -56 to -20 X 10(-9) m2V-1s-1 and chloride as the ion modelling the effect of the sample matrix.

引用

页码：677 / 682

页数：6

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