QUANTITATIVE AMINO-ACID-ANALYSIS OF SUBNANOGRAM LEVELS OF PROTEIN BY OPEN TUBULAR LIQUID-CHROMATOGRAPHY

被引：17

作者：

OATES, MD ^{[1
]}

JORGENSON, JW ^{[1
]}

机构：

[1] UNIV N CAROLINA,DEPT CHEM,CHAPEL HILL,NC 27599

来源：

ANALYTICAL CHEMISTRY | 1990年 / 62卷 / 15期

关键词：

D O I：

10.1021/ac00214a007

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method is described for the hydrolysis and quantitative amino acid analysis of as little as 0.1 ng (4 fmol) of protein. Hydrolysis is performed by using a gas-phase method. The resulting amino acids are derivatized with naphthalene-2, 3-dlcarboxaldehyde and then analyzed by open tubular liquid chromatography with amperometric detection. The total volume present after derivatization is approximately 25 nL. To our knowledge this is currently the lowest level of protein to be quantitatively analyzed, as well as the smallest volume in which derivatization has been accomplished. It was possible to quantitatively determine 14 amino acids with an error of 5.0% for a known protein and 8.5% for a protein whose identity was unknown to the researcher. © 1990, American Chemical Society. All rights reserved.

引用

页码：1577 / 1580

页数：4

共 23 条

[11] PNEUMATIC MICROSYRINGE FOR USE AS AN INJECTOR IN OPEN TUBULAR LIQUID-CHROMATOGRAPHY AND AS A DISPENSER IN MICROANALYSIS [J].