APPLICATION OF INEXPENSIVE EQUIPMENT FOR HIGH-PRESSURE LIQUID-CHROMATOGRAPHY TO ASSAYS FOR TAURINE, GAMMA-AMINO BUTYRIC-ACID, AND 5-HYDROXYTRYPTOPHAN

被引：61

作者：

MEEK, JL ^{[1
]}

机构：

[1] ST ELIZABETHS HOSP, NIMH, LAB PRECLIN PHARMACOL, WASHINGTON, DC 20032 USA

来源：

ANALYTICAL CHEMISTRY | 1976年 / 48卷 / 02期

关键词：

D O I：

10.1021/ac60366a037

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An inexpensive pump is described for high pressure liquid chromatography which can be assembled from readily available parts. This pressurized tank type apparatus can be used with conventional liquid chromatography detectors, or with the spectrophotometers or fluorometers available in most laboratories. The system was applied to measurement of specific amino acids in rat brain tissue: taurine, .gamma.-aminobutyric acid and 5-hydroxytryptophan. The assays, which can measure less than 50 pmol, require only 3-7 min/sample and require no sample preparation other than precipitation of proteins. The apparatus can perform complex separations for analytical work, but its simplicity, high speed and ease of sample preparation make it also suitable for enzymatic and clinical studies.

引用

页码：375 / 379

页数：5

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