CLONING, SEQUENCING AND ENHANCED EXPRESSION OF THE TRICHODERMA-REESEI ENDOXYLANASE-II (PI 9) GENE XLN2

被引:56
作者
SAARELAINEN, R
PALOHEIMO, M
FAGERSTROM, R
SUOMINEN, PL
NEVALAINEN, KMH
机构
[1] Research Laboratories, Alko Ltd., Helsinki, SF-00101
来源
MOLECULAR AND GENERAL GENETICS | 1993年 / 241卷 / 5-6期
关键词
TRICHADERMA REESEI; XYLANASE; GENE SEQUENCE; EXPRESSION;
D O I
10.1007/BF00279891
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Trichoderma reesei xln2 gene coding for the pI9.0 endoxylanase was isolated from the wild-type strain QM6a. The gene contains one intron of 108 nucleotides and codes for a protein of 223 amino acids in which two putative N-glycosylation target sites were found. Three:e different T. reesei strains were transformed by targeting a construct composed of the xln2 gene, including its promoter, to the endogenous cbh1 locus. Highest overall production levels of xylanase were obtained using T. reesei ALK02721, a genetically engineered strain, as a host. Integration into the cbh1 locus was not required for enhanced expression under control of the xln2 promoter.
引用
收藏
页码:497 / 503
页数:7
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