MICROGLIAL LOCALIZATION OF ALPHA-INTERFERON RECEPTOR IN HUMAN BRAIN-TISSUES

被引：42

作者：

YAMADA, T

YAMANAKA, L

机构：

[1] Department of Neurology, Chiba University, Chuo-ku, Chiba, 260, 1-8-1, Inohana

来源：

NEUROSCIENCE LETTERS | 1995年 / 189卷 / 02期

关键词：

ALPHA-INTERFERON; ALPHA-INTERFERON RECEPTOR; MICROGLIA; MACROPHAGE; HUMAN BRAIN;

D O I：

10.1016/0304-3940(95)11452-3

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The localization of the alpha-interferon receptor (alpha-IFNR) was examined in human brain tissues from neurologically normal, Alzheimer's disease (AD) and cerebral infarction cases, using one antibody (IFNaR3) which preferentially recognizes alpha 2-IFN receptor and a second antibody (anti-p40) which recognizes the soluble alpha-IFN alpha/beta receptor In all cases, microglial cells were stained with the antibodies, In infarct brains, macrophages were intensely labeled for alpha-IFNR. The anti-p40 antibody also showed capillary staining which might be due to residual blood plasma. On Western blots, IFNaR3 recognized only a 210 kDa alpha-IFN/receptor complex, indicating its localization in microglial cells. The anti-p40 antibody recognized this complex as well as four other major materials of lower molecular weight. These results suggest that alpha-IFNR protein is constitutively expressed in microglial cells.

引用

页码：73 / 76

页数：4

共 19 条

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