CHARACTERISTICS OF HEPATITIS-B SURFACE-ANTIGEN PRODUCED IN YEAST

被引：7

作者：

CHOO, KB ^{[1
]}

WU, SM ^{[1
]}

LEE, HH ^{[1
]}

LO, SJ ^{[1
]}

机构：

[1] NATL YANG MING MED COLL, GRAD INST MICROBIOL & IMMUNOL, TAIPEI, TAIWAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1985年 / 131卷 / 01期

关键词：

D O I：

10.1016/0006-291X(85)91784-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have constructed an expression plasmid for regulated expression of the hepatitis B surface antigen gene in yeast using promoter of the yeast Pho5 gene. In the yeast transformants, the monomeric HBsAg (22K dalton) was estimated to constitute approximately 3% of the total proteins. On extraction, the HbsAg was found to have a bouyant density of 1.18 g/ml and an Sw.20 value of 54. Electron microscopy revealed particles of heterogeneous size ranging from 18-28 nm. When the yeast HBsAg was used to immunize guinea pigs, the anti-HBsAg antibodies produced could react with human serum HBsAg.

引用

页码：160 / 166

页数：7