A KINETIC PARTITIONING MODEL OF SELECTIVE BINDING OF NONNATIVE PROTEINS BY THE BACTERIAL CHAPERONE SECB

被引：204

作者：

HARDY, SJS ^{[1
]}

RANDALL, LL ^{[1
]}

机构：

[1] WASHINGTON STATE UNIV,DEPT BIOCHEM & BIOPHYS,PULLMAN,WA 99164

来源：

SCIENCE | 1991年 / 251卷 / 4992期

关键词：

D O I：

10.1126/science.1989077

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

An in vitro assay for the interaction of SecB, a molecular chaperone from Escherichia coli, with polypeptide ligands was established based on the ability of SecB to block the refolding of denatured maltose-binding protein. Competition experiments show that SecB binds selectively to nonnative proteins with high affinity and without specificity for a particular sequence of amino acids. It is proposed that selectivity in binding is due to a kinetic partitioning of polypeptides between folding and association with SecB.

引用

页码：439 / 443

页数：5

共 56 条

[21] GROE HEAT-SHOCK PROTEINS PROMOTE ASSEMBLY OF FOREIGN PROKARYOTIC RIBULOSE BISPHOSPHATE CARBOXYLASE OLIGOMERS IN ESCHERICHIA-COLI [J].

GOLOUBINOFF, P ;

GATENBY, AA ;

LORIMER, GH .

NATURE, 1989, 337 (6202) :44-47

[22]

GROARKE JM, 1983, J BIOL CHEM, V258, P2952

[23] QUANTITATIVE TREATMENT OF KINETICS OF FOLDING TRANSITION OF RIBONUCLEASE-A [J].

HAGERMAN, PJ ;

BALDWIN, RL .

BIOCHEMISTRY, 1976, 15 (07) :1462-1473

[24]

HARTL FU, IN PRESS CELL

[25]

HIRS CHW, 1960, J BIOL CHEM, V235, P633

[26] INTERACTIONS OF MISFOLDED INFLUENZA-VIRUS HEMAGGLUTININ WITH BINDING-PROTEIN (BIP) [J].

HURTLEY, SM ;

BOLE, DG ;

HOOVERLITTY, H ;

HELENIUS, A ;

COPELAND, CS .

JOURNAL OF CELL BIOLOGY, 1989, 108 (06) :2117-2126

[27] HEAVY-CHAIN BINDING-PROTEIN RECOGNIZES ABERRANT POLYPEPTIDES TRANSLOCATED INVITRO [J].

KASSENBROCK, CK ;

GARCIA, PD ;

WALTER, P ;

KELLY, RB .

NATURE, 1988, 333 (6168) :90-93

[28] SPECIFIC INTERMEDIATES IN THE FOLDING REACTIONS OF SMALL PROTEINS AND THE MECHANISM OF PROTEIN FOLDING [J].

KIM, PS ;

BALDWIN, RL .

ANNUAL REVIEW OF BIOCHEMISTRY, 1982, 51 :459-489

[29]

KUMAMOTO CA, 1988, J BIOL CHEM, V263, P11554

[30] EVIDENCE FOR SPECIFICITY AT AN EARLY STEP IN PROTEIN EXPORT IN ESCHERICHIA-COLI [J].

KUMAMOTO, CA ;

BECKWITH, J .

JOURNAL OF BACTERIOLOGY, 1985, 163 (01) :267-274

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