TOXICITY TO CHRYSOMELA-TREMULAE (COLEOPTERA, CHRYSOMELIDAE) OF TRANSGENIC POPLARS EXPRESSING A CYSTEINE PROTEINASE-INHIBITOR

被引：168

作者：

LEPLE, JC

BONADEBOTTINO, M

AUGUSTIN, S

PILATE, G

LETAN, VD

DELPLANQUE, A

CORNU, D

JOUANIN, L

机构：

[1] INRA,AMELIORAT ARBRES FORESTIERS STN,F-45160 ARDON,FRANCE

[2] STATE UNIV GHENT,INST NATL RECH AGRON,B-9000 GHENT,BELGIUM

[3] STATE UNIV GHENT,GENET LAB,B-9000 GHENT,BELGIUM

[4] STATE UNIV GHENT,ASSOCIE LAB,B-9000 GHENT,BELGIUM

[5] INRA,BIOL CELLULAIRE LAB,F-78026 VERSAILLES,FRANCE

[6] INRA,ZOOL FORESTIERE STN,F-45160 ARDON,FRANCE

来源：

MOLECULAR BREEDING | 1995年 / 1卷 / 04期

关键词：

CYSTEINE PROTEINASE INHIBITOR; COLEOPTERA; INSECT DIGESTIVE PROTEASE; TRANSGENIC POPLAR;

D O I：

10.1007/BF01248409

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The aim of this study was to test the potential of proteinase inhibitors to control Chrysomela tremulae, a beetle that causes severe damage in young plantations and in short-rotation intensive culture (SRIC) of poplar. As a first step, cysteine proteinases were determined to be the major digestive proteinases of C. tremulae and oryzacystatin OCI, a cysteine proteinase inhibitor, was shown to inhibit this activity in vitro. The gene encoding OCI was introduced into poplar (Populus tremula x P. tremuloides) and transgenic plants expressing OCI at a high level were selected. Feeding tests on these transgenic plants demonstrate the toxicity of OCI-producing poplar leaves against C. tremulae larvae.

引用

页码：319 / 328

页数：10

共 43 条

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