PRODUCTION OF POLY-BETA-HYDROXYBUTYRATE BY FED-BATCH CULTURE OF RECOMBINANT ESCHERICHIA-COLI

被引：71

作者：

KIM, BS

LEE, SY

CHANG, HN

机构：

[1] KOREA ADV INST SCI & TECHNOL,BIOPROC ENGN RES CTR,TAEJON 305701,SOUTH KOREA

[2] KOREA ADV INST SCI & TECHNOL,DEPT CHEM ENGN,TAEJON 305701,SOUTH KOREA

来源：

BIOTECHNOLOGY LETTERS | 1992年 / 14卷 / 09期

关键词：

D O I：

10.1007/BF01029144

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A recombinant Escherichia coli strain harboring the PHB biosynthesis genes from Alcaligenes eutrophus was used to produce poly-beta-hydroxybutyrate (PHB) by pH-stat fed-batch culture. Initial glucose concentration for optimal growth was found to be 20 g/L from a series of flask cultures. A final PHB concentration of 88.8 g/L could be obtained after 42 hrs of cultivation.

引用

页码：811 / 816

页数：6

共 14 条

[1] RAPID GAS-CHROMATOGRAPHIC METHOD FOR DETERMINATION OF POLY-BETA-HYDROXYBUTYRIC ACID IN MICROBIAL BIOMASS [J].