ATOMIC-ABSORPTION SPECTROMETRIC DETECTION OF BIOTIN-CYMANTRENE AS A METALLO-TRACER FOR THE AVIDIN-BIOTIN SYSTEM

被引：10

作者：

REMY, I ^{[1
]}

BROSSIER, P ^{[1
]}

机构：

[1] FAC PHARM DIJON,UNITE IMMUNOANAL,7 BD JEANNE ARC,F-21033 DIJON,FRANCE

来源：

ANALYST | 1993年 / 118卷 / 08期

关键词：

AVIDIN; BIOTIN; CYMANTRENE; IMMUNOASSAY; ZEEMAN ATOMIC ABSORPTION SPECTROMETER;

D O I：

10.1039/an9931801021

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Biotin-cyclopentadienylmanganese tricarbonyl (biotin-cymantrene; biotin-Cy) is proposed as a universal metallo-tracer for immunoassays. Associated with the possibility of detecting this type of label (organometallic moieties) along with different analytical procedures, this reagent appears to be a universal system with the advantages of being inexpensive, stable and directly detected (no substrate is required). The optimum conditions for detecting the organometallic label biotin-Cy with a Zeeman atomic absorption spectrometer and the affinity of biotin-Cy for the streptavidin ligand immobilized on the walls of microtitre plates, wells or tubes are described. The results obtained demonstrate the possibility of assaying a wide range of clinical parameters because the detection limit of biotin-Cy is about 1 pg per assay. Based on the competition between immobilized bovine serum albumin adsorbed on the wall and free bovine serum albumin for specific polyclonal antibodies, the streptavidin-metallo-biotin system was tested on a competitive immunoassay model and a satisfactory calibration graph was obtained.

引用

页码：1021 / 1025

页数：5

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