RECOVERY OF PROTEINS ON A MILLIGRAM SCALE FROM POLYACRYLAMIDE ELECTROPHORESIS GELS, EXEMPLIFIED BY PURIFICATION OF A RETINOL-BINDING PROTEIN

被引：13

作者：

OFVERSTEDT, LG ^{[1
]}

SUNDELIN, J ^{[1
]}

JOHANSSON, G ^{[1
]}

机构：

[1] UNIV UPPSALA,DEPT CELL RES,WALLENBERG LAB,S-75122 UPPSALA,SWEDEN

来源：

ANALYTICAL BIOCHEMISTRY | 1983年 / 134卷 / 02期

关键词：

D O I：

10.1016/0003-2697(83)90310-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：361 / 367

页数：7

共 12 条

[1] TRANSFER OF PROTEINS ACROSS MEMBRANES .1. PRESENCE OF PROTEOLYTICALLY PROCESSED AND UNPROCESSED NASCENT IMMUNOGLOBULIN LIGHT-CHAINS ON MEMBRANE-BOUND RIBOSOMES OF MURINE MYELOMA [J].

BLOBEL, G ;

DOBBERSTEIN, B .

JOURNAL OF CELL BIOLOGY, 1975, 67 (03) :835-851

[2] AMINO-ACID-COMPOSITION OF PROTEINS ELUTED FROM POLYACRYLAMIDE GELS - BACKGROUND CONSIDERATIONS [J].

BROWN, WE ;

HOWARD, GC .

ANALYTICAL BIOCHEMISTRY, 1980, 101 (02) :294-298

[3]

CHRAMBACH A, 1979, ELECTROKINETIC SEPAR, P337

[4] HIGH-PRESSURE LIQUID-CHROMATOGRAPHIC IDENTIFICATION OF PHENYLTHIOHYDANTOIN DERIVATIVES OF ALL 20 COMMON AMINO-ACIDS [J].

FOHLMAN, J ;

RASK, L ;

PETERSON, PA .

ANALYTICAL BIOCHEMISTRY, 1980, 106 (01) :22-26

[5] SCANNING OF POLYACRYLAMIDE-GEL ELECTROPHORESIS COLUMNS FOR DETECTION OF UNSTAINED PROTEIN ZONES AND FOR THEIR LOCALIZATION RELATIVE TO ENZYME-ACTIVITIES [J].

FRIES, E ;

HJERTEN, S .

ANALYTICAL BIOCHEMISTRY, 1975, 64 (02) :466-476

[6] POLYACRYLAMIDE-GEL ELECTROPHORESIS - RECOVERY OF NON-STAINED AND STAINED PROTEINS FROM GEL SLICES [J].

HJERTEN, S ;

LIU, ZQ ;

ZHAO, SL .

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 1983, 7 (02) :101-113

[7] PURIFICATION AND CHARACTERIZATION OF 2 FORMS OF A LOW-AFFINITY CA2+-ATPASE FROM ERYTHROCYTE-MEMBRANES [J].

HJERTEN, S ;

PAN, H .

BIOCHIMICA ET BIOPHYSICA ACTA, 1983, 728 (02) :281-288

[8]

HJERTEN S, 1962, ARCH BIOCHEM BIOPHYS, P147

[9]

NEVILLE DM, 1971, J BIOL CHEM, V246, P6328

[10] PROTEIN-CONCENTRATION AND RECOVERY FROM GEL SLABS BY DISPLACEMENT ELECTROPHORESIS (ISOTACHOPHORESIS) AND THE EFFECTS OF ELCTROOSMOSIS AND COUNTER FLOW [J].

OFVERSTEDT, LG ;

JOHANSSON, G ;

FROMAN, G ;

HJERTEN, S .

ELECTROPHORESIS, 1981, 2 (03) :168-173

← 1 2 →