INTERLEUKIN-8 AND RANTES INHIBIT BASOPHIL HISTAMINE-RELEASE INDUCED WITH MONOCYTE CHEMOTACTIC AND ACTIVATING FACTOR MONOCYTE CHEMOATTRACTANT PEPTIDE-1 AND HISTAMINE RELEASING-FACTOR

被引:37
作者
ALAM, R
FORSYTHE, PA
LETTBROWN, MA
GRANT, JA
机构
[1] Department of Medicine, University of Texas Medical Branch, Galveston
关键词
D O I
10.1165/ajrcmb/7.4.427
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The objective of this study was to investigate the effect of interleukin-8 (IL-8) and RANTES on basophil histamine release induced with monocyte chemoattractant peptide-1 (MCP-1) and crude histamine releasing factor (HRF). IL-8 induced low levels of histamine release (8.5 +/- 0.5%) from basophils obtained from only six of 20 donors at high concentrations (10(-6) M). RANTES induced histamine release (16 +/- 2%) from basophils of four of 15 donors at 10(-7) M concentration. However, both IL-8 and RANTES inhibited MCP-1- and HRF-induced histamine release from basophils dose-dependently at concentrations of 10(-9) to 10(-7) M. Basophils from all donors showed a significant inhibitory response (> 15%). The maximal inhibition of MCP-1 and HRF by IL-8 was 28 +/- 4% and 48 +/- 8%, respectively. The maximal inhibition of MCP-1 and HRF by RANTES was 26 +/- 4% and 43 +/- 6%, respectively. Peripheral blood mononuclear cell-derived HRF was purified into three distinct peaks by reverse-phase high performance liquid chromatography. Peak I contained MCP-1 as judged by binding to an immunoaffinity column that was prepared with anti-MCP-1 antibody. IL-8 inhibited histamine release induced with all three peaks of HRF. The inhibition of histamine release by IL-8 was significantly higher in normal subjects than in allergic patients (59 +/- 9 % versus 31 +/- 7%, P < 0.05). Both IL-8 and RANTES inhibited cytokine-induced histamine release only and did not affect histamine release by anti-IgE, FMLP, and C5a. Our results suggest that IL-8 and RANTES are potent inhibitors of cytokine-induced histamine release from basophils.
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页码:427 / 433
页数:7
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