DNA integrity as a biomarker of marine pollution: Strand breaks in seastar (Asterias rubens) and Dab (Limanda limanda)

被引:38
作者
Everaarts, JM
机构
[1] Department of Chemical Oceanography and Marine Pollution, Netherlands Institute for Sea Research, 1790 AB Den Burg-Texel
关键词
D O I
10.1016/0025-326X(95)00145-D
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
DNA strand breaks in seastars and dab were measured by the time-dependent partial alkaline unwinding of DNA followed by the determination of the double-stranded to total DNA ratio (F-value), Highest DNA integrity (0.75 < F < 0.85) was found in seastars from offshore reference sites, whereas lowest integrity (0.35 < F < 0.55) was identified in specimens from the coastal zone and certain expected uncontaminated offshore areas. A significant correlation existed between the fraction of double-stranded DNA and the concentration of low chlorinated biphenyl congeners and with H atoms substituted in the meta and para position of the biphenyl skeleton, Over 90% of double-stranded DNA was measured in dab obtained from pristine areas, with an average F-value in specimens from most sampling stations varying from 0.75 to 0.85, during August/September 1991. Samples taken in May/June 1992 showed significantly lower DNA integrity (0.55 < F < 0.70 in most stations). A significant decrease of the integrity was established with increase in concentration of the congeners (tri to hepta chloro substituted and with or without a H atom substituted in the meta and para position).
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页码:431 / 438
页数:8
相关论文
共 33 条
[11]  
EVERAARTS JM, UNPUB ECOTOXICOLOGY
[12]  
EVERAARTS JM, 1989, NIOZ19896 REP, P1
[13]   SINGLE-STAGE CLEAN-UP OF ANIMAL TISSUE EXTRACTS FOR ORGANOCHLORINE RESIDUE ANALYSIS [J].
HOLDEN, AV ;
MARSDEN, K .
JOURNAL OF CHROMATOGRAPHY, 1969, 44 (3-4) :481-&
[14]  
KANTER PM, 1982, MOL PHARMACOL, V22, P145
[15]   ON THE DISTRIBUTION OF POLYCHLORINATED BIPHENYL CONGENERS AND HEXACHLOROBENZENE IN DIFFERENT TISSUES OF DAB (LIMANDA-LIMANDA) FROM THE NORTH-SEA [J].
KNICKMEYER, R ;
STEINHART, H .
CHEMOSPHERE, 1989, 19 (8-9) :1309-1320
[17]   USING THE DNA ALKALINE UNWINDING ASSAY TO DETECT DNA DAMAGE IN LABORATORY AND ENVIRONMENTALLY EXPOSED CELLS AND TISSUES [J].
NACCI, D ;
JACKIM, E .
MARINE ENVIRONMENTAL RESEARCH, 1989, 28 (1-4) :333-337
[18]   POPULATION BIOLOGY OF DAB LIMANDA-LIMANDA IN THE SOUTHEASTERN NORTH-SEA [J].
RIJNSDORP, AD ;
VETHAAK, AD ;
VANLEEUWEN, PI .
MARINE ECOLOGY PROGRESS SERIES, 1992, 91 (1-3) :19-35
[19]   COMPLETE CHARACTERIZATION OF POLYCHLORINATED BIPHENYL CONGENERS IN COMMERCIAL AROCLOR AND CLOPHEN MIXTURES BY MULTIDIMENSIONAL GAS-CHROMATOGRAPHY ELECTRON-CAPTURE DETECTION [J].
SCHULZ, DE ;
PETRICK, G ;
DUINKER, JC .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 1989, 23 (07) :852-859
[20]  
SHUGART L, 1992, SETAC SP P, P125