TRANSFORMATION OF TRICHODERMA-VIRIDE USING THE NEUROSPORA-CRASSA-PYR4 GENE AND ITS USE IN THE EXPRESSION OF A TAKA-AMYLASE-A GENE FROM ASPERGILLUS-ORYZAE

被引:9
作者
CHENG, C [1 ]
TSUKAGOSHI, N [1 ]
UDAKA, S [1 ]
机构
[1] NAGOYA UNIV,FAC AGR,NAGOYA,AICHI 464,JAPAN
关键词
Expression of the Taka-amylase A gene; pyr4 gene of Neurospora crassa; Transformation of Trichoderma viride;
D O I
10.1007/BF00309916
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
A pyrG mutant of Trichoderma viride, a very efficient cellulase producer, was isolated from among 5-fluoroorotic acid-resistant mutants. The mutation was complemented with the pyr4 gene of Neurospora crassa and used as a selection marker for the transformation of T. viride. A plasmid vector, pDJB1-Taa, carrying both the pyr4 gene and a gene encoding Taka-amylase A from Aspergillus oryzae, was constructed and introduced into protoplasts of T. viride pyrG-. The transformation frequency was 1-10 transformants (3 on average) per μg DNA. One transformant showed highly elevated α-amylase production (about 17 times higher than the recipient level) and the integration of more than one copy of the Taka-amylase gene. © 1990 Springer-Verlag.
引用
收藏
页码:453 / 456
页数:4
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