TRANSFORMATION OF TRICHODERMA-VIRIDE USING THE NEUROSPORA-CRASSA-PYR4 GENE AND ITS USE IN THE EXPRESSION OF A TAKA-AMYLASE-A GENE FROM ASPERGILLUS-ORYZAE

被引：9

作者：

CHENG, C ^{[1
]}

TSUKAGOSHI, N ^{[1
]}

UDAKA, S ^{[1
]}

机构：

[1] NAGOYA UNIV,FAC AGR,NAGOYA,AICHI 464,JAPAN

来源：

CURRENT GENETICS | 1990年 / 18卷 / 05期

关键词：

Expression of the Taka-amylase A gene; pyr4 gene of Neurospora crassa; Transformation of Trichoderma viride;

D O I：

10.1007/BF00309916

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A pyrG mutant of Trichoderma viride, a very efficient cellulase producer, was isolated from among 5-fluoroorotic acid-resistant mutants. The mutation was complemented with the pyr4 gene of Neurospora crassa and used as a selection marker for the transformation of T. viride. A plasmid vector, pDJB1-Taa, carrying both the pyr4 gene and a gene encoding Taka-amylase A from Aspergillus oryzae, was constructed and introduced into protoplasts of T. viride pyrG-. The transformation frequency was 1-10 transformants (3 on average) per μg DNA. One transformant showed highly elevated α-amylase production (about 17 times higher than the recipient level) and the integration of more than one copy of the Taka-amylase gene. © 1990 Springer-Verlag.

引用

页码：453 / 456

页数：4

共 14 条

[1] DEVELOPMENT OF A HIGH-FREQUENCY TRANSFORMING VECTOR FOR ASPERGILLUS-NIDULANS [J].