LOW-COST DEVICE FOR ELECTROTRANSFORMATION AND ITS APPLICATION TO THE HIGHLY EFFICIENT TRANSFORMATION OF ESCHERICHIA-COLI AND ENTEROCOCCUS-FAECALIS

被引：23

作者：

FUJIMOTO, S

HASHIMOTO, H

IKE, Y

机构：

[1] Department of Microbiology, School of Medicine, Gunma University, Maebashi

来源：

PLASMID | 1991年 / 26卷 / 02期

关键词：

D O I：

10.1016/0147-619X(91)90053-Y

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A simple, low cost device for electrotransformation has been designed and constructed. The cost of the circuit was only $150. Maximum field strength of 12,000 V/cm with an actual time constant up to 11 msec was obtained with a newly designed circuitry and a 0.1-cm electrode gap cuvette. Eschericia coli strains DH1, DH5α, and LE392 were transformed at an efficiency of 109/μg DNA with plasmids pUC18 and pBR322. E. faecalis strain OG1X was transformed at an efficiency of 0.9 × 105/μg DNA without treatment with glycine. © 1991.

引用

页码：131 / 135

页数：5

共 16 条

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