EXPRESSION OF THE HPRT GENE DURING SPERMATOGENESIS - IMPLICATIONS FOR SEX-CHROMOSOME INACTIVATION

被引:25
作者
SHANNON, M
HANDEL, MA
机构
[1] UNIV TENNESSEE, DEPT ZOOL, KNOXVILLE, TN 37996 USA
[2] UNIV TENNESSEE, PROGRAM CELLULAR MOLEC & DEV BIOL, KNOXVILLE, TN 37996 USA
关键词
D O I
10.1095/biolreprod49.4.770
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The goal of this study was to determine the developmental pattern of expression of the X-linked gene for hypoxanthine phosphoribosyltransferase (Hprt) during spermatogenesis and the relevance of this expression to X-chromosome inactivation during meiotic prophase. The results demonstrated that HPRT activity is maintained in mouse spermatogenic cells throughout development in spite of X-chromosome inactivation; however, specific activities of HPRT in meiotic and postmeiotic germ cells were significantly lower than in premeiotic ones. Maintenance of Hprt transcripts all stages was also demonstrated. Interestingly, the highest level of Hprt transcripts was found in leptotene/zygotene spermatocytes, suggesting a hyperactivation of the Hprt gene and/or stabilization of Hprt transcripts in these cells. Hprt transcripts were present at very low levels in pachytene spermatocytes, and at slightly elevated levels in round spermatids. It was also found that the relative abundance of Hprt transcripts in the somatic cells of germ-cell-deficient testes was much greater than that in meiotic and postmeiotic germ cells, even though their activities of HPRT were similar. Examination of the translational status of Hprt transcripts in testicular cells revealed that while most of the transcript was translationally active in somatic cells of testes, less than half of the transcript was on polysomes in pachytene spermatocytes and round spermatids. Since no functional autosomal Hprt gene exists in the mouse, these data suggest that the germ cell maintains both transcript and protein product of the Hprt gene in spite of apparent X-chromosome inactivation.
引用
收藏
页码:770 / 778
页数:9
相关论文
共 41 条
[32]   THE 2 CANDIDATE TESTIS-DETERMINING-Y GENES (ZFY-1 AND ZFY-2) ARE DIFFERENTIALLY EXPRESSED IN FETAL AND ADULT-MOUSE TISSUES [J].
NAGAMINE, CM ;
CHAN, K ;
HAKE, LE ;
LAU, YFC .
GENES & DEVELOPMENT, 1990, 4 (01) :63-74
[33]   SEPARATION OF MOUSE SPERMATOGENIC CELLS BY SEDIMENTATION-VELOCITY - MORPHOLOGICAL CHARACTERIZATION [J].
ROMRELL, LJ ;
BELLVE, AR ;
FAWCETT, DW .
DEVELOPMENTAL BIOLOGY, 1976, 49 (01) :119-131
[35]   MEASUREMENT BY QUANTITATIVE PCR OF CHANGES IN HPRT, PGK-1, PGK-2, APRT, MTASE, AND ZFY GENE TRANSCRIPTS DURING MOUSE SPERMATOGENESIS [J].
SINGERSAM, J ;
ROBINSON, MO ;
BELLVE, AR ;
SIMON, MI ;
RIGGS, AD .
NUCLEIC ACIDS RESEARCH, 1990, 18 (05) :1255-1259
[36]   BEHAVIOR OF XY PAIR IN MAMMALS [J].
SOLARI, AJ .
INTERNATIONAL REVIEW OF CYTOLOGY-A SURVEY OF CELL BIOLOGY, 1974, 38 :273-317
[37]   EXPRESSION OF CELLULAR PROTOONCOGENES IN THE MOUSE MALE GERM LINE - A DISTINCTIVE 2.4-KILOBASE PIM-1 TRANSCRIPT IS EXPRESSED IN HAPLOID POSTMEIOTIC CELLS [J].
SORRENTINO, V ;
MCKINNEY, MD ;
GIORGI, M ;
GEREMIA, R ;
FLEISSNER, E .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (07) :2191-2195
[38]   THE EXPRESSION PATTERN OF THE PYRUVATE-DEHYDROGENASE E1-ALPHA SUBUNIT GENES DURING SPERMATOGENESIS IN ADULT-MOUSE [J].
TAKAKUBO, F ;
DAHL, HHM .
EXPERIMENTAL CELL RESEARCH, 1992, 199 (01) :39-49
[39]   HYBRIDIZATION OF DENATURED RNA AND SMALL DNA FRAGMENTS TRANSFERRED TO NITROCELLULOSE [J].
THOMAS, PS .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (09) :5201-5205
[40]  
White MJD, 1973, ANIMAL CYTOLOGY EVOL