EXPRESSION AND PURIFICATION OF RECOMBINANT MAREKS-DISEASE VIRUS SEROTYPE-1 SPECIFIC PHOSPHORYLATED PROTEIN-PP38 IN ESCHERICHIA-COLI

被引：11

作者：

ENDOH, D

NIIKURA, M

HIRAI, K

INAGAKI, N

HAYASHI, M

NAKAJIMA, K

SATO, F

机构：

[1] UNIV TOKYO,FAC AGR,DEPT MICROBIOL,BUNKYO KU,TOKYO 113,JAPAN

[2] HOKKAIDO UNIV,FAC VET MED,ANIM SCI LAB,SAPPORO,HOKKAIDO 060,JAPAN

[3] TOKYO MED & DENT UNIV,MED RES INST,DEPT CELL REGULAT,DIV VIROL & IMMUNOL,BUNKYO KU,TOKYO 113,JAPAN

[4] NATL RES INST AQUACULTURE,FISHERIES AGCY,PATHOL SECT & FISH PATHOL,NAKATSUHAMA NANSEI,MIE 4221,JAPAN

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 1994年 / 56卷 / 05期

关键词：

MAREKS DISEASE VIRUS; M21 MONOCLONAL ANTIBODY; NI-TAG; PHOSPHORYLATED PROTEIN; RECOMBINANT PP38;

D O I：

10.1292/jvms.56.823

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Phosphorylated protein pp38 is the only protein that is detected in the Marek's disease (MD) lymphoma caused by MD virus serotype 1 (MDV-1) and lymphoblastoid cell lines. In this study, a recombinant protein coded for by the almost entire open reading frame of the MDV-1 pp38 cDNA was produced in E. coli and purified by affinity chromatography. Prior to the expression and purification of the protein, cDNA containing the entire coding region for pp38 was cloned and its nucleotide sequence was determined. Immunoblot analysis indicated that the expressed recombinant protein electrophoresed close to that of the pp38 in infected cells. The difference in mobility of the purified recombinant and the pp38 in infected cells corresponded to a fusion peptide. The recombinant pp38 may be of interest for function analyses and the diagnotic use of pp38.

引用

页码：823 / 826

页数：4

共 21 条

[1] LOSS OF MAREKS-DISEASE VIRUS TUMORIGENICITY IS ASSOCIATED WITH TRUNCATION OF RNAS TRANSCRIBED WITHIN BAMHI-H [J].