DETERMINATION OF AMPICILLIN IN HUMAN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING ULTRAVIOLET DETECTION

被引：14

作者：

AKHTAR, MJ ^{[1
]}

KHAN, S ^{[1
]}

KHAN, MAS ^{[1
]}

机构：

[1] WELLCOME PAKISTAN LTD,RES & DEV LABS,D-43,SITE,KARACHI,PAKISTAN

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1993年 / 11卷 / 4-5期

关键词：

AMPICILLIN; REVERSED-PHASE HPLC; ISOCRATIC ELUTION; UV DETECTION; HUMAN PLASMA;

D O I：

10.1016/0731-7085(93)80031-U

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatographic method with isocratic elution has been developed for the determination of ampicillin in human plasma. The method comprises injection of the plasma after protein precipitation on to a reversed-phase C18 column, using phosphate buffer (pH 3.5)-methanol as the mobile phase. The method involves UV detection at 225 nm. This method provides a simple technique for the rapid analysis of ampicillin within the concentration range 0.2-25 mug ml-1 with a high degree of accuracy and precision.

引用

页码：375 / 378

页数：4