APPLICATION OF BETA-CYCLODEXTRIN FOR THE ANALYSIS OF ESTROGENIC STEROIDS IN HUMAN URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：17

作者：

LAMPARCZYK, H ^{[1
]}

ZARZYCKI, PK ^{[1
]}

NOWAKOWSKA, J ^{[1
]}

OCHOCKA, RJ ^{[1
]}

机构：

[1] MED ACAD GDANSK,FAC PHARM,PL-80416 GDANSK,POLAND

来源：

CHROMATOGRAPHIA | 1994年 / 38卷 / 3-4期

关键词：

COLUMN LIQUID CHROMATOGRAPHY; BETA-CYCLODEXTRIN IN MOBILE PHASE; ESTROGENS; PREGNANT AND NONPREGNANT WOMEN;

D O I：

10.1007/BF02290331

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A procedure is described for simultaneous determination of estriol, estrone and 17 beta-estradiol in human urine. After acid hydrolysis of the sulphate conjugates, the estrogens were extracted into diethyl ether. The ether extracts were concentrated and applied directly to an HPLC column. Using a 25 cm C-18 column and acetonitrile-water modified by the addition of beta-cyclodextrin as mobile phase, the separation of estriol, estradiol and estrone was achieved within 20 minutes. The extraction of estrogens from the biological matrix is excellent. Estrogens were detected using a UV-detector (280 nm) or a spectrofluorimetric detector (lambda(exc) = 280 nm, lambda(em) = 312 nm). The latter detection system has been used for the determination of estrogens in the urine of non-pregnant women. The procedure is simple and can be used in clinical practice.

引用

页码：168 / 172

页数：5

共 15 条

[1] MEASUREMENT OF URINARY ESTRIOL GLUCURONIDES DURING THE MENSTRUAL-CYCLE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].