CUCUMBER MOSAIC-VIRUS GENOME IS ENCAPSIDATED IN ALFALFA MOSAIC-VIRUS COAT PROTEIN EXPRESSED IN TRANSGENIC TOBACCO PLANTS

被引:23
作者
CANDELIERHARVEY, P [1 ]
HULL, R [1 ]
机构
[1] JOHN INNES CTR,JOHN INNES INST,VIRUS RES DEPT,NORWICH RES PK,COLNEY LANE,NORWICH NR4 7UH,ENGLAND
关键词
AIMV COAT PROTEIN; TRANSGENIC PLANT; CMV HETEROENCAPSIDATION; IC PCR; RISK;
D O I
10.1007/BF01968840
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The expression of viral coat protein (CP) in transgenic plants has been shown to be very effective in virus plant protection. However, the introduction of CP genes into plants presents the potential risk of the encapsidation of a superinfecting viral genome in the transgenic protein, an event which could change the epidemiology of the disease. To detect the potential heterologous encapsidation of the cucumber mosaic virus (CMV) genome by alfalfa mosaic virus (AlMV) CP expressed in transgenic tobacco plants, a system of immunocapture (IC) and amplification by polymerase chain reaction (PCR) was optimized. This provided high sensitivity and reliable selection of the heterologously encapsidated CMV genome in the presence of natural CMV particles. As little as 2 pg of virus could be detected by immunocapture/polymerase chain reaction (IC/PCR) technique. Evidence for heterologous encapsidation of the CMV genome was found in 11 of the 33 transgenic plants tested two weeks after CMV inoculation. This demonstrates a significant rate of heterologous encapsidation events between two unrelated viruses in transgenic plants. Since CP is involved in the interactions of the virus particle with its vector, the release in the field of such transgenic plants could alter the transmission properties of some important viruses.
引用
收藏
页码:277 / 285
页数:9
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