IONIC MECHANISMS INVOLVED IN THE SECRETORY EFFECTS OF HISTAMINE IN THE RAT ADRENAL-MEDULLA

Histamine activation of H, receptors elicited the release of adrenaline from in vitro perfused rat adrenal with an EC50 of 3 AM. Neither Na+ deprivation nor complete membrane depolarization abolished the histamine-mediated secretory response but it was partially dependent on extracellular Ca2+. Nitrendipine and BAY-K-8644 affected the release induced by histamine concentrations at over 3 muM. Delayed application of histamine pulses, after external Ca2+ removal, led to a decline in to a plateau at 50% of the initial release. Pretreatment with ionomycin abolished this Ca2+ deprivation-resistant component. These data suggest that secretion evoked by low concentrations of histamine occurs by mobilization of Ca2+ from internal stores whereas higher concentrations use Ca2+ from both intracellular and extracellular sources.