CELLULASE GENES OF CELLULOMONAS-UDA CB4 .1. CLONING AND EXPRESSION OF BETA-GLUCOSIDASE GENES IN ESCHERICHIA-COLI

被引：12

作者：

NAKAMURA, K ^{[1
]}

MISAWA, N ^{[1
]}

KITAMURA, K ^{[1
]}

机构：

[1] KIRIN BREWERY CO LTD, APPL BIOSCI LAB, TAKASAKI, GUNMA 37012, JAPAN

来源：

JOURNAL OF BIOTECHNOLOGY | 1986年 / 3卷 / 04期

关键词：

D O I：

10.1016/0168-1656(86)90006-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two .beta.-glucosidase genes in Cellulomonas uda CB4 were cloned in Escherichia coli with pAT325 constructed from pAT153 and pBR325. Plasmids pCC1 and pCG1 were isolated from the transformants producing .beta.-glucosidase, and the .beta.-glucosidase genes cloned were in 6.1 and 8.1 kb BamHI fragments, respectively. The amount of .beta.-glucosidase expressed in E. coli harboring pCC1 and pCG1 was 1.2 and 4.0 times that in the present strain. E. coli harboring pCC1 grew efficiently on cellobiose.

引用

页码：239 / 246

页数：8

共 13 条

[11] CLONING OF ASPERGILLUS-NIGER GENES IN YEAST - EXPRESSION OF THE GENE CODING ASPERGILLUS BETA-GLUCOSIDASE [J].

PENTTILA, ME ;

NEVALAINEN, KMH ;

RAYNAL, A ;

KNOWLES, JKC .

MOLECULAR & GENERAL GENETICS, 1984, 194 (03) :494-499

[12] CLONING AND EXPRESSION OF THE STRUCTURAL GENE FOR BETA-GLUCOSIDASE OF KLUYVEROMYCES-FRAGILIS IN ESCHERICHIA-COLI AND SACCHAROMYCES-CEREVISIAE [J].

RAYNAL, A ;

GUERINEAU, M .

MOLECULAR & GENERAL GENETICS, 1984, 195 (1-2) :108-115

[13] DETECTION OF SPECIFIC SEQUENCES AMONG DNA FRAGMENTS SEPARATED BY GEL-ELECTROPHORESIS [J].

SOUTHERN, EM .

JOURNAL OF MOLECULAR BIOLOGY, 1975, 98 (03) :503-+

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