INVITRO AGING OF BETA-AMYLOID PROTEIN CAUSES PEPTIDE AGGREGATION AND NEUROTOXICITY

被引：819

作者：

PIKE, CJ

WALENCEWICZ, AJ

GLABE, CG

COTMAN, CW

机构：

[1] UNIV CALIF IRVINE,DEPT PSYCHOBIOL,IRVINE,CA 92717

[2] UNIV CALIF IRVINE,DEPT MOLEC BIOL,IRVINE,CA 92717

[3] UNIV CALIF IRVINE,DEPT BIOCHEM,IRVINE,CA 92717

来源：

BRAIN RESEARCH | 1991年 / 563卷 / 1-2期

关键词：

ALZHEIMERS DISEASE; BETA-AMYLOID; SENILE PLAQUE; NEUROTOXIC; AGGREGATION;

D O I：

10.1016/0006-8993(91)91553-D

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

beta-Amyloid peptide forms the senile plaques of Alzheimer's disease and has been previously demonstrated to have both trophic and toxic effects on neurons in vitro. We report here that synthetic beta-amyloid peptide shows both aggregation and neurotoxicity after a 2-4 day incubation period, but is neurite-promoting and not toxic in its initially solubilized state. SDS-PAGE characterization shows that newly solubilized beta-amyloid is predominantly monomeric whereas incubated peptide has several high molecular weight species.

引用

页码：311 / 314

页数：4

共 21 条

[11] BETA-AMYLOID PROTEIN INCREASES THE VULNERABILITY OF CULTURED CORTICAL-NEURONS TO EXCITOTOXIC DAMAGE
KOH, JY
YANG, LL
COTMAN, CW
[J]. BRAIN RESEARCH, 1990, 533 (02) : 315 - 320
[12] AMYLOID PLAQUE CORE PROTEIN IN ALZHEIMER-DISEASE AND DOWN SYNDROME
MASTERS, CL
SIMMS, G
WEINMAN, NA
MULTHAUP, G
MCDONALD, BL
BEYREUTHER, K
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (12) : 4245 - 4249
[13] PIKE CJ, IN PRESS EUR J PHARM
[14] BETA-AMYLOID FROM ALZHEIMER-DISEASE BRAINS INHIBITS SPROUTING AND SURVIVAL OF SYMPATHETIC NEURONS
ROHER, AE
BALL, MJ
BHAVE, SV
WAKADE, AR
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 174 (02) : 572 - 579
[15] EVIDENCE THAT BETA-AMYLOID PROTEIN IN ALZHEIMERS-DISEASE IS NOT DERIVED BY NORMAL PROCESSING
SISODIA, SS
KOO, EH
BEYREUTHER, K
UNTERBECK, A
PRICE, DL
[J]. SCIENCE, 1990, 248 (4954) : 492 - 495
[16] IDENTIFICATION OF CELLULAR AND EXTRACELLULAR SITES OF AMYLOID PRECURSOR PROTEIN EXTRACYTOPLASMIC DOMAIN IN NORMAL AND ALZHEIMER-DISEASE BRAINS
TATEOSTROFF, B
MAJOCHA, RE
MAROTTA, CA
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (02) : 745 - 749
[17] BETA-AMYLOID PROTEIN PROMOTES NEURITIC BRANCHING IN HIPPOCAMPAL CULTURES
WHITSON, JS
GLABE, CG
SHINTANI, E
ABCAR, A
COTMAN, CW
[J]. NEUROSCIENCE LETTERS, 1990, 110 (03) : 319 - 324
[18] AMYLOID-BETA PROTEIN ENHANCES THE SURVIVAL OF HIPPOCAMPAL-NEURONS INVITRO
WHITSON, JS
SELKOE, DJ
COTMAN, CW
[J]. SCIENCE, 1989, 243 (4897) : 1488 - 1490
[19] NEUROTOXICITY OF A FRAGMENT OF THE AMYLOID PRECURSOR ASSOCIATED WITH ALZHEIMERS-DISEASE
YANKNER, BA
DAWES, LR
FISHER, S
VILLAKOMAROFF, L
OSTERGRANITE, ML
NEVE, RL
[J]. SCIENCE, 1989, 245 (4916) : 417 - 420
[20] NEUROTROPHIC AND NEUROTOXIC EFFECTS OF AMYLOID BETA-PROTEIN - REVERSAL BY TACHYKININ NEUROPEPTIDES
YANKNER, BA
DUFFY, LK
KIRSCHNER, DA
[J]. SCIENCE, 1990, 250 (4978) : 279 - 282

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