ROLE OF A RAS HOMOLOG IN THE LIFE-CYCLE OF SCHIZOSACCHAROMYCES POMBE

被引：234

作者：

FUKUI, Y

KOZASA, T

KAZIRO, Y

TAKEDA, T

YAMAMOTO, M

机构：

[1] UNIV TOKYO, INST MED SCI, DEPT CHEM, PO TAKANAWA, TOKYO 108, JAPAN

[2] UNIV TOKYO, INST MED SCI, MOLEC GENET LAB, TOKYO 108, JAPAN

来源：

CELL | 1986年 / 44卷 / 02期

关键词：

D O I：

10.1016/0092-8674(86)90767-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have analyzed the function of the only ras homolog in S. pombe detectable by Southern blotting, ras1, which is homologous to mammalian ras genes and has been cloned. We have disrupted the ras1 gene and have replaced it with ras1Val17, which corresponds to a transforming variant of mammalian ras. Loss of ras1 activity by disruption results in the complete inability to mate. The cell body of a ras1- strain is extensively deformed, and a ras1-/ras1- diploid sporulates very poorly. Unlike RAS1 and RAS2 of S. cerevisiae, ras1 of S. pombe appears to have no effect on adenylate cyclase activity. This suggests that the target enzymes presumably modulated by ras proteins in signal transduction are not the same for all organisms.

引用

页码：329 / 336

页数：8

共 57 条

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