THE TONB PROTEIN OF YERSINIA-ENTEROCOLITICA AND ITS INTERACTIONS WITH TONB-BOX PROTEINS

被引:37
作者
KOEBNIK, R
BAUMLER, AJ
HEESEMANN, J
BRAUN, V
HANTKE, K
机构
[1] UNIV TUBINGEN,LEHRSTUHL MIKROBIOL 2,AUF MORGENSTELLE 28,W-7400 TUBINGEN 1,GERMANY
[2] INST HYG & MIKROBIOL,W-8700 WURZBURG,GERMANY
来源
MOLECULAR & GENERAL GENETICS | 1993年 / 237卷 / 1-2期
关键词
YERSINIA-ENTEROCOLITICA; TONB GENE; TOLA GENE; COLICIN UPTAKE; IRON TRANSPORT;
D O I
10.1007/BF00282796
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The tonB gene is required for energy-dependent transport processes across the outer membrane of gram-negative bacteria. Using the antibiotics albomycin and ferrimycin, a tonB mutant of Yersinia enterocolitica was isolated. Comparison of the tonB mutant with the parent strain revealed that in Y. enterocolitica the uptake of ferrioxamine, ferrichrome, pesticin and heme is TonB-dependent. The tonB gene from Y. enterocolitica was sequenced and found to be similar to those of other Enterobacteria. The Y. enterocolitica tonB gene complemented a Y. enterocolitica tonB mutant. In contrast, some TonB functions of an Escherichia coli tonB mutant were not restored by the tonB gene of Y. enterocolitica. The observed differences in the ability to complement E. coli TonB functions correlated with the degree to which the TonB boxes of the receptors and colicins differed from the TonB box consensus sequence. Furthermore, the N-terminal membrane anchor of the TonB proteins and the TolA protein are likely to form an alpha-helix with an identical sequence motif (SHLS) located at one face of the alpha-helix, suggesting this region to be involved in the functional cross-talk between the TonB-ExbBD- and TolABQR-dependent transport systems across the outer membrane.
引用
收藏
页码:152 / 160
页数:9
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