NOVEL METHOD OF ISOLATING TAXANES FROM CELL-SUSPENSION CULTURES OF YEW (TAXUS SPP)

被引：22

作者：

KETCHUM, REB

GIBSON, DM

机构：

[1] USDA, Agricultural Research Service, Plant Protection Research Unit, US Plant, Soil, and Nutrition Laboratory, Ithaca, New York 14882, Tower Road

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1995年 / 18卷 / 06期

关键词：

D O I：

10.1080/10826079508009278

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is described to isolate a mixture of taxanes from cell suspension cultures of Taxus species. The aqueous suspension medium is pre-filtered and centrifuged to remove cellular debris, and then passed through either nylon or PVDF membranes. Contaminants are washed from the membranes and the taxanes are eluted with appropriate solvents. This method provides a rapid, efficient, and inexpensive means of extracting taxanes from cell suspension medium, as well as a significant reduction in the total volume of solvents used.

引用

页码：1093 / 1111

页数：19

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