NUCLEOTIDE-SEQUENCE OF A CDNA FOR MOUSE SQUALENE EPOXIDASE

被引：21

作者：

KOSUGA, K

HATA, S

OSUMI, T

SAKAKIBARA, J

ONO, T

机构：

[1] HIMEJI INST TECHNOL,FAC SCI,DEPT LIFE SCI,HIMEJI,HYOGO 67812,JAPAN

[2] NIIGATA UNIV,SCH MED,DEPT BIOCHEM,NIIGATA 951,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1995年 / 1260卷 / 03期

关键词：

SQUALENE EPOXIDASE; CHOLESTEROL BIOSYNTHESIS; CDNA CLONING; FAD-BINDING MOTIF; (MOUSE LIVER);

D O I：

10.1016/0167-4781(94)00245-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A full-length cDNA encoding mouse squalene epoxidase was isolated by screening a mouse liver cDNA library with the rat squalene epoxidase gene as a probe. The cDNA had an open reading frame for a 572 amino acid polypeptide with a calculated molecular mass of 63.8 kDa. The predicted amino acid sequence of the mouse enzyme contained an FAD-binding motif, and was 93% identical to that of the rat enzyme. The former is one amino acid shorter than the latter. Blotting analyses showed that the mRNA is 2.8 kb in size and that a single copy of the gene is present in the mouse genome.

引用

页码：345 / 348

页数：4