INITIATION OF MEIOSIS AND SPORULATION IN SACCHAROMYCES-CEREVISIAE REQUIRES A NOVEL PROTEIN-KINASE HOMOLOG

被引：100

作者：

YOSHIDA, M ^{[1
]}

KAWAGUCHI, H ^{[1
]}

SAKATA, Y ^{[1
]}

KOMINAMI, K ^{[1
]}

HIRANO, M ^{[1
]}

SHIMA, H ^{[1
]}

AKADA, R ^{[1
]}

YAMASHITA, I ^{[1
]}

机构：

[1] HIROSHIMA UNIV,CTR GENE SCI,HIROSHIMA 724,JAPAN

来源：

MOLECULAR & GENERAL GENETICS | 1990年 / 221卷 / 02期

关键词：

Meiosis; Northern hybridization; Regulatory circuit; Sporulation; Yeast;

D O I：

10.1007/BF00261718

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

SME1 was cloned due to its high copy number effect: it enabled MATα/MATα diploid cells to undergo meiosis and sporulation in a vegetative medium. Disruption of SME1 resulted in a recessive Spo- phenotype. These results suggest that SME1 is a positive regulator for meiosis. DNA sequencing analysis revealed an open reading frame of 645 amino acids. An amino terminal peptide of ca 400 amino acids in the deduced protein was similar to known protein kinases. Transcription of SME1 was regulated negatively by nitrogen and glucose and positively by MATα/MATα and IME1, another positive regulator gene of meiosis. By complementation analysis, SME1 was found to be identical to IME2, which had been shown to be important in meiosis. These results suggest that IME1 product stimulates meiosis by activating transcription of SME1 (IME2) and that protein phosphorylation is required for initiation of meiosis. © 1990 Springer-Verlag.

引用

页码：176 / 186

页数：11

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